Structure analysis of the global metabolic regulator Crc from Pseudomonas aeruginosa
文献类型：期刊论文
| 作者 | Wei Y(魏勇); Zhang H(张衡); Gao ZQ(高增强); Wei, Y; Zhang, H; Gao, ZQ; Xu, JH; Liu, QS; Dong, YH; Xu JH(徐建华); Liu QS(刘全胜); Dong YH(董宇辉) |
| 刊名 | IUBMB LIFE
 |
| 出版日期 | 2013 |
| 卷号 | 65期号:SI1页码:50-57 |
| 关键词 | global metabolic regulator Pseudomonas aeruginosa RNA binding crystal structure target for antimicrobials |
| 英文摘要 | The global metabolic regulator catabolite repression control (Crc) has recently been found to modulate the susceptibility to antibiotics and virulence in the opportunistic pathogen Pseudomonas aeruginosa and been suggested as a nonlethal target for novel antimicrobials. In P. aeruginosa, Crc couples with the CA motifs from the small RNA CrcZ to form a post-transcriptional regulator system and is removed from the 5'-end of the target mRNAs. In this study, we first reported the crystal structure of Crc from P. aeruginosa refined to 2.20 angstrom. The structure showed that it consists of two halves with similar overall topology and there are 11 beta strands surrounded by 13 helices, forming a four-layered a/beta-sandwich. The circular dichroism spectroscopy revealed that it is thermostable in solution and shares similar characteristics to that in crystal. Comprehensive structural analysis and comparison with the homologies of Crc showed high similarity with several known nucleases and consequently may be classified into a member exodeoxyribonuclease III. However, it shows distinct substrate specificity (RNA as the preferred substrate) compared to these DNA endonucleases. Structural comparisons also revealed potential RNA recognition and binding region mainly consisting of five flexible loops. Our structure study provided the basis for the future application of Crc as a target to develop new antibiotics. (c) 2012 IUBMB Life, 65(1):5057, 2013 |
| 学科主题 | Biochemistry & Molecular Biology; Cell Biology |
| 收录类别 | SCI |
| WOS记录号 | WOS:000312886200008 |
| 公开日期 | 2016-05-03 |
| 源URL | [http://ir.ihep.ac.cn/handle/311005/223798]  |
| 专题 | 中国科学院高能物理研究所 |
| 推荐引用方式 GB/T 7714 | 魏勇；张衡；高增强；Wei, Y,Zhang, H,Gao, ZQ,et al. Structure analysis of the global metabolic regulator Crc from Pseudomonas aeruginosa[J]. IUBMB LIFE,2013,65(SI1):50-57. |
| APA | 魏勇；张衡；高增强；Wei, Y.,Zhang, H.,Gao, ZQ.,Xu, JH.,Liu, QS.,...&徐建华；刘全胜；董宇辉.(2013).Structure analysis of the global metabolic regulator Crc from Pseudomonas aeruginosa.IUBMB LIFE,65(SI1),50-57. |
| MLA | 魏勇；张衡；高增强；Wei, Y,et al."Structure analysis of the global metabolic regulator Crc from Pseudomonas aeruginosa".IUBMB LIFE 65.SI1(2013):50-57. |
入库方式： OAI收割
来源：高能物理研究所
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