Shape evolution with temperature of a thermotolerant protein (PeaT1) in solution detected by small angle X-ray scattering
文献类型：期刊论文
| 作者 | Xing XQ(邢雪青) ; Xing, XQ; Wang W(王维); Liu, Q; Wang, W; Zhang, KH; Li, T; Cai, Q; Mo, G; Cheng, WD
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| 刊名 | PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS
 |
| 出版日期 | 2013 |
| 卷号 | 81期号:1页码:53-62 |
| 关键词 | domain structure restoration structure prediction SRCD SAXS PeaT1 |
| 英文摘要 | The protein elicitor from Alternaria tenuissima (PeaT1) presented excellent thermotolerance and potential application in agriculture as a pesticide. Previous synchrotron radiation circular dichroism study demonstrated that the secondary structures in PeaT1 protein are reversible with temperature change. To further clarify the mechanism of its thermotolerance, synchrotron radiation small angle x-ray scattering (SAXS) technique was used to study the shape change of PeaT1 protein with temperature in this article. Ab initio structure restorations based on the SAXS data revealed that PeaT1 protein has a prolate shape with a P2 symmetry axis along the prolate anisometric direction. With temperature increase, a gooseneck vase-like (25 degrees C), to jug-like (55 degrees C), then to oval (85 degrees C) shape change can be found, and these shape changes are also approximately reversible with temperature decrease. PeaT1 protein contains two homogenous molecules, and each of them consists of F, NAC, T, and UBA domains. The structures of the four domains were predicted. Simulated annealing algorithm was used to superimpose the domain structures onto the SAXS shapes. It was found that all the structural domains have position rotation and translation with temperature change, but the NAC domains are relatively stable, playing a role of frame. This shape change information provides clues for further exploring its biological function and application. Proteins 2013. (c) 2012 Wiley Periodicals, Inc. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 收录类别 | SCI |
| WOS记录号 | WOS:000312551700004 |
| 公开日期 | 2016-05-03 |
| 源URL | [http://ir.ihep.ac.cn/handle/311005/223803]  |
| 专题 | 高能物理研究所_多学科研究中心 |
| 推荐引用方式 GB/T 7714 | Xing XQ,Xing, XQ,Wang W,et al. Shape evolution with temperature of a thermotolerant protein (PeaT1) in solution detected by small angle X-ray scattering[J]. PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS,2013,81(1):53-62. |
| APA | 邢雪青.,Xing, XQ.,王维.,Liu, Q.,Wang, W.,...&张坤浩；蔡泉；默广；程伟东；王德红；宫宇；陈中军；吴忠华.(2013).Shape evolution with temperature of a thermotolerant protein (PeaT1) in solution detected by small angle X-ray scattering.PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS,81(1),53-62. |
| MLA | 邢雪青,et al."Shape evolution with temperature of a thermotolerant protein (PeaT1) in solution detected by small angle X-ray scattering".PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS 81.1(2013):53-62. |
入库方式： OAI收割
来源：高能物理研究所
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