Chain Elongation and Cyclization in Type III PKS DpgA
文献类型：期刊论文
| 作者 | 吴海臣;Wu, HC; Li, YS; Liu, YC; Lyu, SY; Wu, CJ; Li, TL |
| 刊名 | CHEMBIOCHEM
 |
| 出版日期 | 2012 |
| 卷号 | 13期号:6页码:862-871 |
| 关键词 | biosynthesis chain elongation enzyme catalysis polyketone cyclization type III polyketide synthase |
| 英文摘要 | Chain elongation and cyclization of precursors of dihydroxyphenylacetyl-CoA (DPA-CoA) catalyzed by the bacterial type III polyketide synthase DpgA were studied. Two labile intermediates, di- and tri-ketidyl-CoA (DK- and TK-CoA), were proposed and chemically synthesized. In the presence of DpgABD, each of these with [13C3]malonyl-CoA (MA-CoA) was able to form partially 13C-enriched DPA-CoA. By NMR and MS analysis, the distribution of 13C atoms in the partially 13C-enriched DPA-CoA shed light on how the polyketide chain elongates and cyclizes in the DpgA-catalyzed reaction. Polyketone intermediates elongate in a manner different from that which had been believed: two molecules of DK-CoA, or one DK-CoA plus one acetoacetyl-CoA (AA-CoA), but not two molecules of AA-CoA can form one molecule of DPA-CoA. As a result, polyketidyl-CoA serves as both the starter and extender, whereas polyketone-CoA without the terminal carboxyl group can only act as an extender. The terminal carboxyl group is crucial for the cyclization that likely takes place on CoA. |
| 学科主题 | Biochemistry & Molecular Biology; Pharmacology & Pharmacy |
| 收录类别 | SCI |
| WOS记录号 | WOS:000302609100016 |
| 公开日期 | 2016-05-03 |
| 源URL | [http://ir.ihep.ac.cn/handle/311005/224072]  |
| 专题 | 高能物理研究所_多学科研究中心 |
| 推荐引用方式 GB/T 7714 | 吴海臣；Wu, HC,Li, YS,Liu, YC,et al. Chain Elongation and Cyclization in Type III PKS DpgA[J]. CHEMBIOCHEM,2012,13(6):862-871. |
| APA | 吴海臣；Wu, HC,Li, YS,Liu, YC,Lyu, SY,Wu, CJ,&Li, TL.(2012).Chain Elongation and Cyclization in Type III PKS DpgA.CHEMBIOCHEM,13(6),862-871. |
| MLA | 吴海臣；Wu, HC,et al."Chain Elongation and Cyclization in Type III PKS DpgA".CHEMBIOCHEM 13.6(2012):862-871. |
入库方式： OAI收割
来源：高能物理研究所
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