中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Chain Elongation and Cyclization in Type III PKS DpgA

文献类型:期刊论文

作者吴海臣;Wu, HC; Li, YS; Liu, YC; Lyu, SY; Wu, CJ; Li, TL
刊名CHEMBIOCHEM
出版日期2012
卷号13期号:6页码:862-871
关键词biosynthesis chain elongation enzyme catalysis polyketone cyclization type III polyketide synthase
英文摘要Chain elongation and cyclization of precursors of dihydroxyphenylacetyl-CoA (DPA-CoA) catalyzed by the bacterial type III polyketide synthase DpgA were studied. Two labile intermediates, di- and tri-ketidyl-CoA (DK- and TK-CoA), were proposed and chemically synthesized. In the presence of DpgABD, each of these with [13C3]malonyl-CoA (MA-CoA) was able to form partially 13C-enriched DPA-CoA. By NMR and MS analysis, the distribution of 13C atoms in the partially 13C-enriched DPA-CoA shed light on how the polyketide chain elongates and cyclizes in the DpgA-catalyzed reaction. Polyketone intermediates elongate in a manner different from that which had been believed: two molecules of DK-CoA, or one DK-CoA plus one acetoacetyl-CoA (AA-CoA), but not two molecules of AA-CoA can form one molecule of DPA-CoA. As a result, polyketidyl-CoA serves as both the starter and extender, whereas polyketone-CoA without the terminal carboxyl group can only act as an extender. The terminal carboxyl group is crucial for the cyclization that likely takes place on CoA.
学科主题Biochemistry & Molecular Biology; Pharmacology & Pharmacy
收录类别SCI
WOS记录号WOS:000302609100016
公开日期2016-05-03
源URL[http://ir.ihep.ac.cn/handle/311005/224072]  
专题高能物理研究所_多学科研究中心
推荐引用方式
GB/T 7714
吴海臣;Wu, HC,Li, YS,Liu, YC,et al. Chain Elongation and Cyclization in Type III PKS DpgA[J]. CHEMBIOCHEM,2012,13(6):862-871.
APA 吴海臣;Wu, HC,Li, YS,Liu, YC,Lyu, SY,Wu, CJ,&Li, TL.(2012).Chain Elongation and Cyclization in Type III PKS DpgA.CHEMBIOCHEM,13(6),862-871.
MLA 吴海臣;Wu, HC,et al."Chain Elongation and Cyclization in Type III PKS DpgA".CHEMBIOCHEM 13.6(2012):862-871.

入库方式: OAI收割

来源:高能物理研究所

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