The structural basis of the response regulator DrRRA from Deinococcus radiodurans
文献类型：期刊论文
| 作者 | Liu Y(刘颖); Gao ZQ(高增强); She Z(佘准); Liu, Y; Gao, ZQ; She, Z; Qu, K; Wang, WJ; Shtykova, EV; Xu, JH |
| 刊名 | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
 |
| 出版日期 | 2012 |
| 卷号 | 417期号:4页码:1206-1212 |
| 关键词 | Deinococcus radiodurans Two component system Response regulator Extensive interdomain interface DNA-binding Crystal structure SAXS |
| 英文摘要 | DrRRA, a vital and recently discovered gene product of Deinococcus radiodurans, is a member of the OmpR/PhoB family of response regulators that couple with the cognate histidine kinase (HK) to form a typical two component system (TCS). It is known that the DrRRA is responsible for the transcriptional levels of numerous genes mostly relating to the stress response and DNA repair. In this paper, the crystal structures of the effector domain and full-length protein of DrRRA with resolutions of 1.6 and 2.3 angstrom, respectively, are determined. These crystal structures depicted that DrRRA has the structural features of the OmpR/PhoB subfamily and were also confirmed by SAXS investigation of the protein in solution. Our data suggest that the receiver domain blocks the binding of DNA to the DNA recognition helix of effector domain; while the interdomain interface would be unwrapped, via the phosphorylation of receiver domain and/or the inducement of DNA, in order to provide DNA binding. (C) 2011 Elsevier Inc. All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 收录类别 | SCI |
| WOS记录号 | WOS:000300196100017 |
| 公开日期 | 2016-05-03 |
| 源URL | [http://ir.ihep.ac.cn/handle/311005/224117]  |
| 专题 | 中国科学院高能物理研究所 |
| 推荐引用方式 GB/T 7714 | Liu Y,Gao ZQ,She Z,et al. The structural basis of the response regulator DrRRA from Deinococcus radiodurans[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2012,417(4):1206-1212. |
| APA | 刘颖.,高增强.,佘准.,Liu, Y.,Gao, ZQ.,...&董宇辉.(2012).The structural basis of the response regulator DrRRA from Deinococcus radiodurans.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,417(4),1206-1212. |
| MLA | 刘颖,et al."The structural basis of the response regulator DrRRA from Deinococcus radiodurans".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 417.4(2012):1206-1212. |
入库方式： OAI收割
来源：高能物理研究所
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