Structural and SAXS analysis of the budding yeast SHU-complex proteins
文献类型：期刊论文
| ; | |
| 作者 | She Z(佘准); Gao ZQ(高增强); Liu Y(刘颖); She, Z; Gao, ZQ; Liu, Y; Wang, WJ; Liu, GF; Shtykova, EV; Xu, JH |
| 刊名 | FEBS LETTERS
 ; FEBS LETTERS
|
| 出版日期 | 2012 ; 2012 |
| 卷号 | 586期号:16页码:2306-2312 |
| 关键词 | DNA repair Protein-complex Paralog SAXS DNA binding DNA repair Protein-complex Paralog SAXS DNA binding |
| DOI | 10.1016/j.febslet.2012.06.024 |
| 英文摘要 | In Saccharomyces cerevisiae, four proteins, Shu1, Shu2, Psy3 and Csm2, form a stable SHU-complex both in vivo and in vitro. These proteins are involved in the early stages of the homologous recombination DNA damage repair process. In this paper, the crystal structure of the Psy3-Csm2 sub-complex is presented at 1.8 angstrom resolution and successfully fitted into our small angle X-ray scattering (SAXS) data of the SHU-complex. Taken together with our electrophoretic mobility shift assay (EMSA) results, a model is proposed for the SHU-protein complex coupled with DNA.; In Saccharomyces cerevisiae, four proteins, Shu1, Shu2, Psy3 and Csm2, form a stable SHU-complex both in vivo and in vitro. These proteins are involved in the early stages of the homologous recombination DNA damage repair process. In this paper, the crystal structure of the Psy3-Csm2 sub-complex is presented at 1.8 angstrom resolution and successfully fitted into our small angle X-ray scattering (SAXS) data of the SHU-complex. Taken together with our electrophoretic mobility shift assay (EMSA) results, a model is proposed for the SHU-protein complex coupled with DNA. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics; Cell Biology ; Biochemistry & Molecular Biology; Biophysics; Cell Biology |
| 收录类别 | SCI |
| WOS记录号 | WOS:000306694800016 ; WOS:000306694800016 |
| 公开日期 | 2016-05-03 |
| 源URL | [http://ir.ihep.ac.cn/handle/311005/224199]  |
| 专题 | 中国科学院高能物理研究所 |
| 推荐引用方式 GB/T 7714 | She Z,Gao ZQ,Liu Y,et al. Structural and SAXS analysis of the budding yeast SHU-complex proteins, Structural and SAXS analysis of the budding yeast SHU-complex proteins[J]. FEBS LETTERS, FEBS LETTERS,2012, 2012,586, 586(16):2306-2312, 2306-2312. |
| APA | 佘准.,高增强.,刘颖.,She, Z.,Gao, ZQ.,...&董宇辉.(2012).Structural and SAXS analysis of the budding yeast SHU-complex proteins.FEBS LETTERS,586(16),2306-2312. |
| MLA | 佘准,et al."Structural and SAXS analysis of the budding yeast SHU-complex proteins".FEBS LETTERS 586.16(2012):2306-2312. |
入库方式： OAI收割
来源：高能物理研究所
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