Live-cell single-molecule imaging reveals clathrin and caveolin-1 dependent docking of SMAD4 at the cell membrane
文献类型：期刊论文
| 作者 | Yang, Y; Wolfram, J; Shen, JL; Zhao, YL; Fang, XH; Shen, HF; Ferrari, M;赵宇亮 |
| 刊名 | FEBS LETTERS
 |
| 出版日期 | 2013 |
| 卷号 | 587期号:24页码:3912-3920 |
| 关键词 | SMAD4 Membrane docking Single-molecule fluorescence imaging Clathrin Caveolin-1 |
| 英文摘要 | Transforming growth factor beta (TGF-beta) signaling is important for many biological processes. Although the sequential events of this cascade are known, the dynamics remain speculative. Here, live-cell single-molecule total internal reflection fluorescence microscopy was used to monitor the dynamics of SMAD4, a TGF-beta downstream effector, in MDA-MB-231 breast cancer cells. Contrary to previous belief, SMAD4 was detectable at the cytoplasmic membrane, displaying two subpopulations with different membrane docking behaviors. These subpopulations were regulated by clathrin and caveolin-1, and had opposing roles in the nuclear shuttling of SMAD4 and the subsequent transcriptional regulation of genes associated with cell migration. The notion that membrane-docking behaviors of downstream molecules could predict the cellular response to growth factors may revolutionize the way we view cell signaling. Structured summary of protein interactions: SMAD4 and caveolin-1 colocalize by fluorescence microscopy (View interaction) Crown Copyright (C) 2013 Published by Elsevier B.V. on behalf of Federation of European Biochemical Society. All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics; Cell Biology |
| 收录类别 | SCI |
| WOS记录号 | WOS:000327400100005 |
| 公开日期 | 2016-05-03 |
| 源URL | [http://ir.ihep.ac.cn/handle/311005/224247]  |
| 专题 | 高能物理研究所_多学科研究中心 |
| 推荐引用方式 GB/T 7714 | Yang, Y,Wolfram, J,Shen, JL,et al. Live-cell single-molecule imaging reveals clathrin and caveolin-1 dependent docking of SMAD4 at the cell membrane[J]. FEBS LETTERS,2013,587(24):3912-3920. |
| APA | Yang, Y.,Wolfram, J.,Shen, JL.,Zhao, YL.,Fang, XH.,...&Ferrari, M；赵宇亮.(2013).Live-cell single-molecule imaging reveals clathrin and caveolin-1 dependent docking of SMAD4 at the cell membrane.FEBS LETTERS,587(24),3912-3920. |
| MLA | Yang, Y,et al."Live-cell single-molecule imaging reveals clathrin and caveolin-1 dependent docking of SMAD4 at the cell membrane".FEBS LETTERS 587.24(2013):3912-3920. |
入库方式： OAI收割
来源：高能物理研究所
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