Solution Small Angle X-ray Scattering (SAXS) Studies of RecQ from Deinococcus radiodurans and Its Complexes with Junction DNA Substrates
文献类型：期刊论文
| 作者 | Wang WJ(王文佳) ; Hu HF(胡海峰); Wang, WJ; Hou, HF; Du, Q; Zhang, W; Liu, GF; Shtykova, EV; Xu, JH; Liu, P
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| 刊名 | JOURNAL OF BIOLOGICAL CHEMISTRY
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| 出版日期 | 2013 |
| 卷号 | 288期号:45页码:32414-32423 |
| 关键词 | DNA Helicase Homologous Recombination Junctions Molecular Modeling X-ray Scattering RecQ SAXS |
| 英文摘要 | RecQ helicases, essential enzymes for maintaining genome integrity, possess the capability to participate in a wide variety of DNA metabolisms. They can initiate the homologous recombination repair pathway by unwinding damaged dsDNA and suppress hyper-recombination by promoting Holliday junction (HJ) migration. To learn how DrRecQ participates in the homologous recombination repair pathway, solution structures of Deinococcus radiodurans RecQ (DrRecQ) and its complexes with DNA substrates were investigated by small angle x-ray scattering. We found that the catalytic core and the most N-terminal HRDC (helicase and RNase D C-terminal) domain (HRDC1) undergo a conformational change to a compact state upon binding to a junction DNA. Furthermore, models of DrRecQ in complexes with two kinds of junction DNA (fork junction and HJ) were built based on the small angle x-ray scattering data, and together with the EMSA results, possible binding sites were proposed. It is demonstrated that two DrRecQ molecules bind to the opposite arms of HJ. This architecture is similar to the RuvAB complex and is hypothesized to be highly conserved in the other HJ migration proteins. This work provides us new clues to understand the roles DrRecQ plays in the RecFOR pathway. |
| 学科主题 | Biochemistry & Molecular Biology |
| 收录类别 | SCI |
| WOS记录号 | WOS:000328681700026 |
| 公开日期 | 2016-05-03 |
| 源URL | [http://ir.ihep.ac.cn/handle/311005/224366]  |
| 专题 | 高能物理研究所_多学科研究中心 |
| 推荐引用方式 GB/T 7714 | Wang WJ,Hu HF,Wang, WJ,et al. Solution Small Angle X-ray Scattering (SAXS) Studies of RecQ from Deinococcus radiodurans and Its Complexes with Junction DNA Substrates[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2013,288(45):32414-32423. |
| APA | 王文佳.,胡海峰.,Wang, WJ.,Hou, HF.,Du, Q.,...&董宇辉.(2013).Solution Small Angle X-ray Scattering (SAXS) Studies of RecQ from Deinococcus radiodurans and Its Complexes with Junction DNA Substrates.JOURNAL OF BIOLOGICAL CHEMISTRY,288(45),32414-32423. |
| MLA | 王文佳,et al."Solution Small Angle X-ray Scattering (SAXS) Studies of RecQ from Deinococcus radiodurans and Its Complexes with Junction DNA Substrates".JOURNAL OF BIOLOGICAL CHEMISTRY 288.45(2013):32414-32423. |
入库方式： OAI收割
来源：高能物理研究所
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