High-resolution crystal structure reveals a HEPN domain at the C-terminal region of S-cerevisiae RNA endonuclease Swt1
文献类型：期刊论文
| 作者 | 彭淑霞;Peng, SX; Zhou, K; Wang, WJ; Gao, ZQ; Dong, YH; Liu, QS |
| 刊名 | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
 |
| 出版日期 | 2014 |
| 卷号 | 453期号:4页码:826-832 |
| 关键词 | HEPN domain Crystal structure RNA endonuclease SAXS Swt1 |
| 英文摘要 | Swt1 is an RNA endonuclease that plays an important role in quality control of nuclear messenger ribonucleoprotein particles (mRNPs) in eukaryotes; however, its structural details remain to be elucidated. Here, we report the crystal structure of the C-terminal (CT) domain of Swt1 from Saccharomyces cerevisiae, which shares common characteristics of higher eukaryotes and prokaryotes nucleotide binding (HEPN) domain superfamily. To study in detail the full-length protein structure, we analyzed the low-resolution architecture of Swt1 in solution using small angle X-ray scattering (SAXS) method. Both the CT domain and middle domain exhibited a good fit upon superimposing onto the molecular envelope of Swt1. Our study provides the necessary structural information for detailed analysis of the functional role of Swt1, and its importance in the process of nuclear mRNP surveillance. (C) 2014 Elsevier Inc. All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 收录类别 | SCI |
| WOS记录号 | WOS:000346329000025 |
| 公开日期 | 2016-05-03 |
| 源URL | [http://ir.ihep.ac.cn/handle/311005/224857]  |
| 专题 | 中国科学院高能物理研究所 |
| 推荐引用方式 GB/T 7714 | 彭淑霞；Peng, SX,Zhou, K,Wang, WJ,et al. High-resolution crystal structure reveals a HEPN domain at the C-terminal region of S-cerevisiae RNA endonuclease Swt1[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2014,453(4):826-832. |
| APA | 彭淑霞；Peng, SX,Zhou, K,Wang, WJ,Gao, ZQ,Dong, YH,&Liu, QS.(2014).High-resolution crystal structure reveals a HEPN domain at the C-terminal region of S-cerevisiae RNA endonuclease Swt1.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,453(4),826-832. |
| MLA | 彭淑霞；Peng, SX,et al."High-resolution crystal structure reveals a HEPN domain at the C-terminal region of S-cerevisiae RNA endonuclease Swt1".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 453.4(2014):826-832. |
入库方式： OAI收割
来源：高能物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。