Conserved residues that modulate protein trans-splicing of Npu DnaE split intein
文献类型：期刊论文
| 作者 | Wu, Q; Gao ZQ(高增强) ; 魏勇;Gao, ZQ; Wei, Y; Ma, GL; Zheng, YC; Dong, YH; Liu, YZ;魏勇; Dong YH(董宇辉)
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| 刊名 | BIOCHEMICAL JOURNAL
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| 出版日期 | 2014 |
| 卷号 | 461期号:2页码:247-255 |
| 关键词 | asparagine cyclization crystal structure Nostoc punctiforme PCC73102 (Npu) split intein |
| 英文摘要 | The first crystal trans-structure of a naturally occurring split intein has been determined for the Npu (Nostoc punctiforme PCC73102) DnaE split intein. Guided by this structure, the residues (N)Arg(50) and (C)Ser(35), well conserved in DnaE split inteins, are identified to be critical in the trans-splicing of Npu DnaE split intein. An in vitro splicing assay demonstrates that (N)Arg(50) and cSer35 play synergistic roles in modulating its intein activity. The C-terminal (c)Asn(36) exhibits two orientations of its side chain and interacts with both (N)Arg(50) and cSer35 through hydrogen bonding. These interactions likely facilitate the cyclization of asparagine in the course of protein splicing. The mutation of either residue reduces intein activity, and correlates with the low activity of the Ssp (Cyanobacterium synechocystis sp. strain PCC6803) DnaE split intein. On the other hand, (N)Arg(50) also forms a hydrogen bond with the highly conserved F-block cAse, thus influencing the N-S acyl shift during N-terminal cleavage. Sequence alignments show that residues (N)Arg(5) and cSer35 are rather conserved in those split inteins that lack a penultimate histidine residue. The conserved non-catalytic residues of split inteins modulate the efficiency of protein trans-splicing by hydrogen-bond interactions with the catalytic residues at the splice junction. |
| 学科主题 | Biochemistry & Molecular Biology |
| 收录类别 | SCI |
| WOS记录号 | WOS:000338489400008 |
| 公开日期 | 2016-05-03 |
| 源URL | [http://ir.ihep.ac.cn/handle/311005/224858]  |
| 专题 | 高能物理研究所_多学科研究中心 |
| 推荐引用方式 GB/T 7714 | Wu, Q,Gao ZQ,魏勇；Gao, ZQ,et al. Conserved residues that modulate protein trans-splicing of Npu DnaE split intein[J]. BIOCHEMICAL JOURNAL,2014,461(2):247-255. |
| APA | Wu, Q.,高增强.,魏勇；Gao, ZQ.,Wei, Y.,Ma, GL.,...&董宇辉.(2014).Conserved residues that modulate protein trans-splicing of Npu DnaE split intein.BIOCHEMICAL JOURNAL,461(2),247-255. |
| MLA | Wu, Q,et al."Conserved residues that modulate protein trans-splicing of Npu DnaE split intein".BIOCHEMICAL JOURNAL 461.2(2014):247-255. |
入库方式： OAI收割
来源：高能物理研究所
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