Energy metabolism analysis reveals the mechanism of inhibition of breast cancer cell metastasis by PEG-modified graphene oxide nanosheets
文献类型：期刊论文
| 作者 | Zhou, T; Zhang, B; Wei, P; Du, YP; Zhou, HJ; Yu, MF; Yan, L; Zhang, WD; Nie, GJ; Chen, CY |
| 刊名 | BIOMATERIALS
 |
| 出版日期 | 2014 |
| 卷号 | 35期号:37页码:9833-9843 |
| 关键词 | PEG-modified graphene oxide Breast cancer cell Metastasis Cytoskeletal assembly Mitochondrial oxidative phosphorylation |
| 英文摘要 | Recent advances in nanomedicine provide promising alternatives for cancer treatment that may improve the survival of patients with metastatic disease. The goal of the present study was to evaluate graphene oxide (GO) as a potential anti-metastatic agent. For this purpose, GO was modified with polyethylene glycol (PEG) to form PEG-modified GO (PEG-GO), which improves its aqueous stability and biocompatibility. We show here that PEG-GO exhibited no apparent effects on the viability of breast cancer cells (MDA-MB-231, MDA-MB-436, and SK-BR-3) or non-cancerous cells (MCF-10A), but inhibited cancer cell migration in vitro and in vivo. Analysis of cellular energy metabolism revealed that PEG-GO significantly impaired mitochondrial oxidative phosphorylation (OXPHOS) in breast cancer cells; however, PEG-GO showed no effect on OXPHOS in non-cancerous cells. To explore the underlying mechanisms, a SILAC (Stable Isotope Labeling by Amino acids in Cell culture) labeling strategy was used to quantify protein expression in PEG-GO-exposed breast cancer versus non-cancerous cells. The results indicated that PEG-GO selectively down-regulated PGC-1 alpha in breast cancer cells and thus modified the expression of diverse energy generation-related proteins, which accounts for the inhibition of OXPHOS. The inhibition of OXPHOS by PEG-GO significantly reduced ATP production and impaired assembly of the F-actin cytoskeleton in breast cancer cells, which is required for the migratory and invasive phenotype of cancer cells. Taken together, these effects of PEG-GO on cancer cell metastasis may allow the development of a new approach to treat metastatic breast cancer. (C) 2014 Elsevier Ltd. All rights reserved. |
| 学科主题 | Engineering; Materials Science |
| 收录类别 | SCI |
| WOS记录号 | WOS:000343639700009 |
| 公开日期 | 2016-05-03 |
| 源URL | [http://ir.ihep.ac.cn/handle/311005/224863]  |
| 专题 | 中国科学院高能物理研究所 |
| 推荐引用方式 GB/T 7714 | Zhou, T,Zhang, B,Wei, P,et al. Energy metabolism analysis reveals the mechanism of inhibition of breast cancer cell metastasis by PEG-modified graphene oxide nanosheets[J]. BIOMATERIALS,2014,35(37):9833-9843. |
| APA | Zhou, T.,Zhang, B.,Wei, P.,Du, YP.,Zhou, HJ.,...&陈春英.(2014).Energy metabolism analysis reveals the mechanism of inhibition of breast cancer cell metastasis by PEG-modified graphene oxide nanosheets.BIOMATERIALS,35(37),9833-9843. |
| MLA | Zhou, T,et al."Energy metabolism analysis reveals the mechanism of inhibition of breast cancer cell metastasis by PEG-modified graphene oxide nanosheets".BIOMATERIALS 35.37(2014):9833-9843. |
入库方式： OAI收割
来源：高能物理研究所
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