Characterization of extracellular polymeric substances in the biofilms of typical bacteria by the sulfur K-edge XANES spectroscopy
文献类型：期刊论文
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| 作者 | Lin, HR; Ye, CS; Lv, L; Zheng, CR; Zhang, SH; Zheng, L; Zhao, YD; Yu, X;郑雷; Zhao YD(赵屹东)
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| 刊名 | JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA
 ; JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA
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| 出版日期 | 2014 ; 2014 |
| 卷号 | 26期号:8页码:1763-1768 |
| 关键词 | Biofilms Characterization EPS Sulfur XANES Biofilms Characterization EPS Sulfur XANES |
| ISSN号 | 1001-0742 |
| DOI | 10.1016/j.jes.2014.06.017 |
| 英文摘要 | A combined approach of physicochemical extraction and sulfur K-edge X-ray absorption near-edge structure (XANES) spectroscopy was applied to characterize the extracellular polymeric substances (EPS) of typical bacterial biofilms in this study. Physicochemical analysis showed variation of the contents of DNA, polysaccharide and protein in different fractions of EPS in different mediums. The sulfur K-edge XANES analysis yielded a variety of spectra. Spectral fitting of the XANES spectra utilizing a large set of model compounds showed that there was more reduced sulfur in both LB-EPS (loosely bound EPS) and TB-EPS (tightly bound EPS) of all the biofilms in LB medium than in R2A medium. More oxidized sulfur was identified in LB-EPS than that in TB-EPS, suggesting different niches and physiological heterogeneity in the biofilms. Our results suggested that the sulfur K-edge XANES can be a useful tool to analyze the sulfur speciation in EPS of biofilms. (C) 2014 The Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences. Published by Elsevier B.V.; A combined approach of physicochemical extraction and sulfur K-edge X-ray absorption near-edge structure (XANES) spectroscopy was applied to characterize the extracellular polymeric substances (EPS) of typical bacterial biofilms in this study. Physicochemical analysis showed variation of the contents of DNA, polysaccharide and protein in different fractions of EPS in different mediums. The sulfur K-edge XANES analysis yielded a variety of spectra. Spectral fitting of the XANES spectra utilizing a large set of model compounds showed that there was more reduced sulfur in both LB-EPS (loosely bound EPS) and TB-EPS (tightly bound EPS) of all the biofilms in LB medium than in R2A medium. More oxidized sulfur was identified in LB-EPS than that in TB-EPS, suggesting different niches and physiological heterogeneity in the biofilms. Our results suggested that the sulfur K-edge XANES can be a useful tool to analyze the sulfur speciation in EPS of biofilms. (C) 2014 The Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences. Published by Elsevier B.V. |
| 学科主题 | Environmental Sciences & Ecology ; Environmental Sciences & Ecology |
| 收录类别 | SCI |
| 电子版国际标准刊号 | 1878-7320 |
| 语种 | 英语 |
| WOS记录号 | WOS:000340738000023 ; WOS:000340738000023 |
| 公开日期 | 2016-05-03 |
| 源URL | [http://ir.ihep.ac.cn/handle/311005/225082]  |
| 专题 | 中国科学院高能物理研究所 |
| 推荐引用方式 GB/T 7714 | Lin, HR,Ye, CS,Lv, L,et al. Characterization of extracellular polymeric substances in the biofilms of typical bacteria by the sulfur K-edge XANES spectroscopy, Characterization of extracellular polymeric substances in the biofilms of typical bacteria by the sulfur K-edge XANES spectroscopy[J]. JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA, JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA,2014, 2014,26, 26(8):1763-1768, 1763-1768. |
| APA | Lin, HR.,Ye, CS.,Lv, L.,Zheng, CR.,Zhang, SH.,...&赵屹东.(2014).Characterization of extracellular polymeric substances in the biofilms of typical bacteria by the sulfur K-edge XANES spectroscopy.JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA,26(8),1763-1768. |
| MLA | Lin, HR,et al."Characterization of extracellular polymeric substances in the biofilms of typical bacteria by the sulfur K-edge XANES spectroscopy".JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA 26.8(2014):1763-1768. |
入库方式： OAI收割
来源：高能物理研究所
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