Structural and functional analysis show that the Escherichia coli uncharacterized protein YjcS is likely an alkylsulfatase
文献类型：期刊论文
| 作者 | Liang, YJ; Gao ZQ(高增强) ; Dong YH(董宇辉); 刘全胜;Gao, ZQ; Dong, YH; Liu, QS
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| 刊名 | PROTEIN SCIENCE
 |
| 出版日期 | 2014 |
| 卷号 | 23期号:10页码:1442-1450 |
| 关键词 | alkylsulfatase hydrolase metalloenzyme X-ray crystallography metallo-beta-lactamase |
| 英文摘要 | Sodium dodecyl sulfate (SDS) is a widely used anionic surfactant in industry and research settings, and is known to have a detrimental effect to the environment. The pathway of SDS degradation by bacteria is initiated by an alkylsulfatase and the oxidized product, 1-dodecanoic acid, subsequently enters into the beta-oxidation pathway and is used as a carbon source. In this work, we solved the crystal structure of Escherichia coli uncharacterized protein YjcS and identified that it belongs to the Type III alkylsulfatase with a signal peptide (residues 1-29) at the N terminus. YjcS hydrolyzed SDS and the double mutant D184N-H185A located in the conserved HXHXDH catalytic motif abolished this activity. |
| 学科主题 | Biochemistry & Molecular Biology |
| 收录类别 | SCI |
| WOS记录号 | WOS:000342210600012 |
| 公开日期 | 2016-05-03 |
| 源URL | [http://ir.ihep.ac.cn/handle/311005/225317]  |
| 专题 | 高能物理研究所_多学科研究中心 |
| 推荐引用方式 GB/T 7714 | Liang, YJ,Gao ZQ,Dong YH,et al. Structural and functional analysis show that the Escherichia coli uncharacterized protein YjcS is likely an alkylsulfatase[J]. PROTEIN SCIENCE,2014,23(10):1442-1450. |
| APA | Liang, YJ,高增强,董宇辉,刘全胜；Gao, ZQ,Dong, YH,&Liu, QS.(2014).Structural and functional analysis show that the Escherichia coli uncharacterized protein YjcS is likely an alkylsulfatase.PROTEIN SCIENCE,23(10),1442-1450. |
| MLA | Liang, YJ,et al."Structural and functional analysis show that the Escherichia coli uncharacterized protein YjcS is likely an alkylsulfatase".PROTEIN SCIENCE 23.10(2014):1442-1450. |
入库方式： OAI收割
来源：高能物理研究所
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