A SXRF method for determining the relative concentration of trace elements in plasma protein affected by cisplatin
文献类型：期刊论文
| 作者 | Liu NQ(刘年庆); Liu PS(刘平生); Wang KJ(王孔江); Xu Q(徐清) ; Liu, NQ; Liu, PS; Wang, KJ; Chen, DF; Zhao, JY; Xu, Q
|
| 刊名 | BIOLOGICAL TRACE ELEMENT RESEARCH
 |
| 出版日期 | 2000 |
| 卷号 | 76期号:3页码:#REF! |
| 关键词 | trace elements mouse plasma protein SXRF gel chromatography cisplatin |
| 通讯作者 | Liu, NQ (reprint author), Acad Sinica, Inst High Energy Phys, Beijing 100080, Peoples R China. |
| 英文摘要 | This article presents an analytical method for the determination of the relative concentrations of trace elements in plasma protein by gel chromatography combined with SXRF (synchrotron radiation X-ray fluorescence). The fraction of plasma protein of male Kunming mice (body weight 24.2 +/- 0.3 g), treated with a cisplatin ip injection at a dose of 10 mg/kg, was obtained by the separation of a Sephadex G-50 column (buffered with ammonium acetate, pH 5.7). The SXRF experiments were performed at the Beijing Electron Positron Collider synchrotron radiation facility. The elements (Pt, S, Ca, Fe, Ni, Cu, Zn, Se, Br, and Sr) in the fraction of the plasma proteins (>22 kDa) were assayed using highly sensitive SXRF. The relative concentrations of elements were calculated by a normalization of Compton scattering intensity around 22 keV, after the normalization for the collection time of the X-ray spectrum and the counting of the ion chamber, and subtracting the contribution of the polycarbonate film for the supporting sample. The determination could prove that the element Pt in plasma was bound with macromolecular protein. Cu and S were present in the fraction of the protein in mice treated with cisplatin increase, and their ratios of treated/control were 1.66 +/- 0.06 and 1.78 +/- 0.33, respectively; Zn decreased to a ratio of 0.78 +/- 0.09. Our results are in agreement with others that cisplatin exposure leads to a marked loss of kidney copper and a moderate rise in kidney zinc. However, this article primarily describes one of the analytical methods used; it does not emphasize the results of the effect of cisplatin on trace elements in plasma protein. |
| 学科主题 | Biochemistry & Molecular Biology; Endocrinology & Metabolism |
| 类目[WOS] | Biochemistry & Molecular Biology ; Endocrinology & Metabolism |
| 收录类别 | SCI |
| WOS记录号 | WOS:000089471700008 |
| 公开日期 | 2016-05-03 |
| 源URL | [http://ir.ihep.ac.cn/handle/311005/227369]  |
| 专题 | 高能物理研究所_多学科研究中心 |
| 推荐引用方式 GB/T 7714 | Liu NQ,Liu PS,Wang KJ,et al. A SXRF method for determining the relative concentration of trace elements in plasma protein affected by cisplatin[J]. BIOLOGICAL TRACE ELEMENT RESEARCH,2000,76(3):#REF!. |
| APA | 刘年庆.,刘平生.,王孔江.,徐清.,Liu, NQ.,...&Xu, Q.(2000).A SXRF method for determining the relative concentration of trace elements in plasma protein affected by cisplatin.BIOLOGICAL TRACE ELEMENT RESEARCH,76(3),#REF!. |
| MLA | 刘年庆,et al."A SXRF method for determining the relative concentration of trace elements in plasma protein affected by cisplatin".BIOLOGICAL TRACE ELEMENT RESEARCH 76.3(2000):#REF!. |
入库方式： OAI收割
来源：高能物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。