Integration of a bio-chip technique with technetium-99m labeling provides zeptomolar sensitivity in liver cancer biomarker detection
文献类型：期刊论文
| 作者 | Ma L(马磊); Tang BC(汤博崇); Yang WJ(杨文江); 刘宇(多); Zhao YL(赵宇亮); Li M(李敏); Ma, L; Tang, BC; Yang, WJ; Liu, Y |
| 刊名 | ANALYTICAL METHODS
 |
| 出版日期 | 2015 |
| 卷号 | 7期号:4页码:1622-1626 |
| 通讯作者 | 赵宇亮 ; 李敏 |
| 英文摘要 | In the present work, we integrated a prepared bio-chip with Tc-99m labeling to improve the immunoassay for cancer biomarker protein detection. Efficient binding of a Tc-99m isotope-labeled secondary antibody to the biomarker protein pre-anchored to the bio-chip was followed by detection of g rays from Tc-99m decay via a Gamma Well Counter. We show that alpha-fetoprotein (AFP), a biomarker of liver cancer, can be detected down to 10(-18) M concentration in a buffer solution with good reproducibility. This ultra-high sensitivity is attributed to the aggregation of the Tc-99m isotope-labeled secondary antibody which greatly amplifies the g rays emitted per AFP binding event. The tendency of the isotope-labeled secondary antibody to aggregate was indicated by dynamic light scattering studies on a rhenium/secondary antibody conjugate, a stable structural analogue of the Tc-99m conjugate. The successful zeptomolar-range detection of cancer biomarkers opens new avenues for early disease diagnoses via integrating bio-chips with isotope-labeling techniques. |
| 学科主题 | Chemistry; Food Science & Technology; Spectroscopy |
| 类目[WOS] | Chemistry, Analytical ; Food Science & Technology ; Spectroscopy |
| 收录类别 | SCI ; EI ; CA |
| WOS记录号 | WOS:000349679900051 |
| 公开日期 | 2016-05-03 |
| 源URL | [http://ir.ihep.ac.cn/handle/311005/228167]  |
| 专题 | 中国科学院高能物理研究所 |
| 推荐引用方式 GB/T 7714 | Ma L,Tang BC,Yang WJ,et al. Integration of a bio-chip technique with technetium-99m labeling provides zeptomolar sensitivity in liver cancer biomarker detection[J]. ANALYTICAL METHODS,2015,7(4):1622-1626. |
| APA | 马磊.,汤博崇.,杨文江.,刘宇.,赵宇亮.,...&Li, M.(2015).Integration of a bio-chip technique with technetium-99m labeling provides zeptomolar sensitivity in liver cancer biomarker detection.ANALYTICAL METHODS,7(4),1622-1626. |
| MLA | 马磊,et al."Integration of a bio-chip technique with technetium-99m labeling provides zeptomolar sensitivity in liver cancer biomarker detection".ANALYTICAL METHODS 7.4(2015):1622-1626. |
入库方式： OAI收割
来源：高能物理研究所
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