A Universal Strategy for Aptamer-Based Nanopore Sensing through Host-Guest Interactions inside -Hemolysin
文献类型：期刊论文
| 作者 | Li T(李婷) ; 刘蕾(多); Li YR(李玉茹); Xie JN(谢佳妮); Wu HC(吴海臣); Li, T; Liu, L; Li, YR; Xie, JN; Wu, HC
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| 刊名 | ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
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| 出版日期 | 2015 |
| 卷号 | 54期号:26页码:7678-7681 |
| 关键词 | aptamers host-guest systems magnetic beads nanopore sensors quantitative determination |
| 通讯作者 | 吴海臣 |
| 英文摘要 | Nanopore emerged as a powerful single-molecule technique over the past two decades, and has shown applications in the stochastic sensing and biophysical studies of individual molecules. Here, we report a versatile strategy for nanopore sensing by employing the combination of aptamers and host-guest interactions. An aptamer is first hybridized with a DNA probe which is modified with a ferrocene< subset of>cucurbit[7]uril complex. The presence of analytes causes the aptamer-probe duplex to unwind and release the DNA probe which can quantitatively produce signature current events when translocated through an -hemolysin nanopore. The integrated use of magnetic beads can further lower the detection limit by approximately two to three orders of magnitude. Because aptamers have shown robust binding affinities with a wide variety of target molecules, our proposed strategy should be universally applicable for sensing different types of analytes with nanopore sensors. |
| 学科主题 | Chemistry |
| 类目[WOS] | Chemistry, Multidisciplinary |
| 收录类别 | SCI ; EI ; CA |
| WOS记录号 | WOS:000356390900018 |
| 公开日期 | 2016-05-03 |
| 源URL | [http://ir.ihep.ac.cn/handle/311005/228413]  |
| 专题 | 高能物理研究所_多学科研究中心 |
| 推荐引用方式 GB/T 7714 | Li T,Liu L,Li YR,et al. A Universal Strategy for Aptamer-Based Nanopore Sensing through Host-Guest Interactions inside -Hemolysin[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,2015,54(26):7678-7681. |
| APA | 李婷.,刘蕾.,李玉茹.,谢佳妮.,吴海臣.,...&Wu, HC.(2015).A Universal Strategy for Aptamer-Based Nanopore Sensing through Host-Guest Interactions inside -Hemolysin.ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,54(26),7678-7681. |
| MLA | 李婷,et al."A Universal Strategy for Aptamer-Based Nanopore Sensing through Host-Guest Interactions inside -Hemolysin".ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 54.26(2015):7678-7681. |
入库方式： OAI收割
来源：高能物理研究所
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