Identification and quantification of seleno-proteins by 2-DE-SR-XRF in selenium-enriched yeasts
文献类型：期刊论文
| 作者 | Zhao JT(赵甲亭) ; Gao YX(高愈希); Peng XM(彭晓敏); Li YY(李云云); Xu XH(徐小晗); Li B(李柏); Dong JC(董俊才); Li YF(李玉锋); Zhao, JT; Pu, YX
|
| 刊名 | JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY
 |
| 出版日期 | 2015 |
| 卷号 | 30期号:6页码:1408-1413 |
| 通讯作者 | 李玉锋 |
| 英文摘要 | A comprehensive approach that can identify and quantify selenium (Se) in seleno-proteins in Se-enriched yeast was developed. The Se-containing compounds in Se-enriched yeast were first extracted and then the fraction of Se-containing proteins in the supernatant was analyzed by 2-dimensional electrophoresis (2-DE) and synchrotron radiation X-ray fluorescence (SR-XRF). The detection limit (DL) of SR-XRF analysis for Se quantification in Se-containing proteins after 2-DE separation was calculated to be 0.20 mu g g(-1), which is suitable for Se quantification in the Se-containing spots present on the 2-D gel. After being scanned by SR-XRF, only spots with a mean Se content exceeding twice the DL of SR-XRF were considered to be seleno-proteins. In this way, a total of 157 Se-containing spots in the gel were visually distinguished. Se contents in all the Se-containing proteins of different molecular weights were quantified. The total Se content on the 2-D gel was calculated to be 126.56 mu g g(-1), which covered most of the seleno-proteins on the 2-D gel. |
| 学科主题 | Chemistry; Spectroscopy |
| 类目[WOS] | Chemistry, Analytical ; Spectroscopy |
| 收录类别 | SCI ; EI ; CA |
| WOS记录号 | WOS:000355559500021 |
| 公开日期 | 2016-05-03 |
| 源URL | [http://ir.ihep.ac.cn/handle/311005/228479]  |
| 专题 | 高能物理研究所_多学科研究中心 高能物理研究所_实验物理中心 |
| 推荐引用方式 GB/T 7714 | Zhao JT,Gao YX,Peng XM,et al. Identification and quantification of seleno-proteins by 2-DE-SR-XRF in selenium-enriched yeasts[J]. JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY,2015,30(6):1408-1413. |
| APA | 赵甲亭.,高愈希.,彭晓敏.,李云云.,徐小晗.,...&Li, YF.(2015).Identification and quantification of seleno-proteins by 2-DE-SR-XRF in selenium-enriched yeasts.JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY,30(6),1408-1413. |
| MLA | 赵甲亭,et al."Identification and quantification of seleno-proteins by 2-DE-SR-XRF in selenium-enriched yeasts".JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY 30.6(2015):1408-1413. |
入库方式： OAI收割
来源：高能物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。