Crystal structure of rhodopsin bound to arrestin by femtosecond X-ray laser
文献类型：期刊论文
| 作者 | Kang, YY; Zhou, XE; Gao, X; He, YZ; Liu, W; Ishchenko, A; Barty, A; Sathish, D; Yefanov, O; Han, GW |
| 刊名 | NATURE
 |
| 出版日期 | 2015 |
| 卷号 | 523期号:7562页码:561-567 |
| 英文摘要 | G-protein-coupled receptors (GPCRs) signal primarily through G proteins or arrestins. Arrestin binding to GPCRs blocks G protein interaction and redirects signalling to numerous G-protein-independent pathways. Here we report the crystal structure of a constitutively active form of human rhodopsin bound to a pre-activated form of the mouse visual arrestin, determined by serial femtosecond X-ray laser crystallography. Together with extensive biochemical and mutagenesis data, the structure reveals an overall architecture of the rhodopsin-arrestin assembly in which rhodopsin uses distinct structural elements, including transmembrane helix 7 and helix 8, to recruit arrestin. Correspondingly, arrestin adopts the pre-activated conformation, with a similar to 20 degrees rotation between the amino and carboxy domains, which opens up a cleft in arrestin to accommodate a short helix formed by the second intracellular loop of rhodopsin. This structure provides a basis for understanding GPCR-mediated arrestin-biased signalling and demonstrates the power of X-ray lasers for advancing the frontiers of structural biology. |
| 学科主题 | Science & Technology - Other Topics |
| 类目[WOS] | Multidisciplinary Sciences |
| 收录类别 | SCI |
| WOS记录号 | WOS:000358655200038 |
| 公开日期 | 2016-05-03 |
| 源URL | [http://ir.ihep.ac.cn/handle/311005/228576]  |
| 专题 | 高能物理研究所_多学科研究中心 |
| 推荐引用方式 GB/T 7714 | Kang, YY,Zhou, XE,Gao, X,et al. Crystal structure of rhodopsin bound to arrestin by femtosecond X-ray laser[J]. NATURE,2015,523(7562):561-567. |
| APA | Kang, YY.,Zhou, XE.,Gao, X.,He, YZ.,Liu, W.,...&董宇辉.(2015).Crystal structure of rhodopsin bound to arrestin by femtosecond X-ray laser.NATURE,523(7562),561-567. |
| MLA | Kang, YY,et al."Crystal structure of rhodopsin bound to arrestin by femtosecond X-ray laser".NATURE 523.7562(2015):561-567. |
入库方式： OAI收割
来源：高能物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。