3D imaging of a rice pollen grain using transmission X-ray microscopy
文献类型：期刊论文
| 作者 | Wang, SX; Wang, DJ; Wu, Q; Gao, K; Wang, ZL; Wu, ZY; Wu ZY(吴自玉)
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| 刊名 | JOURNAL OF SYNCHROTRON RADIATION
 |
| 出版日期 | 2015 |
| 卷号 | 22页码:1091-1095 |
| 关键词 | transmission X-ray microscopy nano-tomography rice pollen grain cellular microstructure |
| 通讯作者 | 吴自玉 |
| 英文摘要 | For the first time, the three-dimensional (3D) ultrastructure of an intact rice pollen cell has been obtained using a full-field transmission hard X-ray microscope operated in Zernike phase contrast mode. After reconstruction and segmentation from a series of projection images, complete 3D structural information of a 35 mu m rice pollen grain is presented at a resolution of similar to 100nm. The reconstruction allows a clear differentiation of various subcellular structures within the rice pollen grain, including aperture, lipid body, mitochondrion, nucleus and vacuole. Furthermore, quantitative information was obtained about the distribution of cytoplasmic organelles and the volume percentage of each kind of organelle. These results demonstrate that transmission X-ray microscopy can be quite powerful for non-destructive investigation of 3D structures of whole eukaryotic cells. |
| 学科主题 | Instruments & Instrumentation; Optics; Physics |
| 类目[WOS] | Instruments & Instrumentation ; Optics ; Physics, Applied |
| 收录类别 | SCI ; EI |
| WOS记录号 | WOS:000357407900028 |
| 公开日期 | 2016-05-03 |
| 源URL | [http://ir.ihep.ac.cn/handle/311005/228621]  |
| 专题 | 高能物理研究所_多学科研究中心 |
| 推荐引用方式 GB/T 7714 | Wang, SX,Wang, DJ,Wu, Q,et al. 3D imaging of a rice pollen grain using transmission X-ray microscopy[J]. JOURNAL OF SYNCHROTRON RADIATION,2015,22:1091-1095. |
| APA | Wang, SX.,Wang, DJ.,Wu, Q.,Gao, K.,Wang, ZL.,...&吴自玉.(2015).3D imaging of a rice pollen grain using transmission X-ray microscopy.JOURNAL OF SYNCHROTRON RADIATION,22,1091-1095. |
| MLA | Wang, SX,et al."3D imaging of a rice pollen grain using transmission X-ray microscopy".JOURNAL OF SYNCHROTRON RADIATION 22(2015):1091-1095. |
入库方式： OAI收割
来源：高能物理研究所
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