Detection of 5-methylcytosine and 5-hydroxymethylcytosine in DNA via host-guest interactions inside alpha-hemolysin nanopores
文献类型：期刊论文
| 作者 | Ceng T(曾涛); 刘蕾(多); Li T(李婷) ; Li YR(李玉茹); Gao J(高娟); Wu HC(吴海臣); Zeng, T; Liu, L; Li, T; Li, YR
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| 刊名 | CHEMICAL SCIENCE
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| 出版日期 | 2015 |
| 卷号 | 6期号:10页码:5628-5634 |
| 通讯作者 | 吴海臣 |
| 英文摘要 | Cytosine methylation and hydroxymethylation are both important epigenetic modifications of DNA in mammalian cells. Therefore, profiling DNA (hydroxy) methylation across the genome is vital for understanding their roles in gene regulation. Here, we report a nanopore-based approach for quick and reliable detection of 5-methylcytosine and 5-hydroxymethylcytosine in DNA at the single-molecule level. The single-stranded DNA containing 5-methylcytosine or 5-hydroxymethylcytosine was first selectively modified on the epigenetic base to attach a host-guest complex. Threading of the modified DNA molecules through a-hemolysin nanopores causes unbinding of the host-guest complex and generates highly characteristic current signatures. Statistical analysis of the signature events affords quantitative information about 5-methylcytosine and 5-hydroxymethylcytosine in DNA. Our results suggest that other DNA modifications could also be detected with the developed method. Furthermore, we anticipate our nanopore sensing strategy to be generally useful in biochemical analysis and to find applications in the early diagnosis of diseases. |
| 学科主题 | Chemistry |
| 类目[WOS] | Chemistry, Multidisciplinary |
| 收录类别 | SCI ; EI ; CA |
| WOS记录号 | WOS:000361212000033 |
| 公开日期 | 2016-05-03 |
| 源URL | [http://ir.ihep.ac.cn/handle/311005/228750]  |
| 专题 | 高能物理研究所_多学科研究中心 高能物理研究所_核技术应用研究中心 |
| 推荐引用方式 GB/T 7714 | Ceng T,Liu L,Li T,et al. Detection of 5-methylcytosine and 5-hydroxymethylcytosine in DNA via host-guest interactions inside alpha-hemolysin nanopores[J]. CHEMICAL SCIENCE,2015,6(10):5628-5634. |
| APA | 曾涛.,刘蕾.,李婷.,李玉茹.,高娟.,...&Wu, HC.(2015).Detection of 5-methylcytosine and 5-hydroxymethylcytosine in DNA via host-guest interactions inside alpha-hemolysin nanopores.CHEMICAL SCIENCE,6(10),5628-5634. |
| MLA | 曾涛,et al."Detection of 5-methylcytosine and 5-hydroxymethylcytosine in DNA via host-guest interactions inside alpha-hemolysin nanopores".CHEMICAL SCIENCE 6.10(2015):5628-5634. |
入库方式： OAI收割
来源：高能物理研究所
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