Design and Synthesis of Near-infrared Fluorescent Probes for Imaging of Biological Nitroxyl
文献类型：期刊论文
| 作者 | Tan, Y; Liu, RC; Zhang, HT; Peltier, R; Lam, YW; Zhu, Q; Hu, Y; Sun, HY; Hu Y(胡毅) |
| 刊名 | SCIENTIFIC REPORTS
 |
| 出版日期 | 2015 |
| 卷号 | 5页码:16979 |
| 英文摘要 | Nitroxyl (HNO), the reduced and protonated form of nitric oxide (NO), has recently been identified as an interesting and important signaling molecule in biological systems. However, research on its biosynthesis and bioactivities are hampered by the lack of versatile HNO detection methods applicable to living cells. In this report, two new near-infrared (NIR) probes were designed and synthesized for HNO imaging in living cells. One of the probes was found to display high sensitivity towards HNO, with up to 67-fold of fluorescence increment after reaction with HNO. The detection limit was determined to be as low as 0.043 mu M. The probe displayed high selectivity towards HNO over other biologically related species including metal ions, reactive oxygen species, reactive nitrogen species and reactive sulfur species. Furthermore, the probe was shown to be suitable for imaging of exogenous and endogenous HNO in living cells. Interestingly, the probe was found to be mainly localized in lysosomes. We envision that the new NIR probe described here will serve as a useful tool for further elucidation of the intricate roles of HNO in living cells. |
| 学科主题 | Science & Technology - Other Topics |
| 类目[WOS] | Multidisciplinary Sciences |
| 收录类别 | SCI |
| WOS记录号 | WOS:000365018100001 |
| 公开日期 | 2016-05-03 |
| 源URL | [http://ir.ihep.ac.cn/handle/311005/228813]  |
| 专题 | 高能物理研究所_多学科研究中心 |
| 推荐引用方式 GB/T 7714 | Tan, Y,Liu, RC,Zhang, HT,et al. Design and Synthesis of Near-infrared Fluorescent Probes for Imaging of Biological Nitroxyl[J]. SCIENTIFIC REPORTS,2015,5:16979. |
| APA | Tan, Y.,Liu, RC.,Zhang, HT.,Peltier, R.,Lam, YW.,...&胡毅.(2015).Design and Synthesis of Near-infrared Fluorescent Probes for Imaging of Biological Nitroxyl.SCIENTIFIC REPORTS,5,16979. |
| MLA | Tan, Y,et al."Design and Synthesis of Near-infrared Fluorescent Probes for Imaging of Biological Nitroxyl".SCIENTIFIC REPORTS 5(2015):16979. |
入库方式： OAI收割
来源：高能物理研究所
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