The effect of secondary structures on the generation of characteristic events during the translocation of DNA hybrid through α-hemolysin
文献类型：期刊论文
| 作者 | Wang; Xingyong; Li YR(李玉茹); Li T(李婷) ; 刘蕾(多); Wu HC(吴海臣); Li; Yuru; Li; Ting
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| 刊名 | SCIENCE CHINA-CHEMISTRY
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| 出版日期 | 2015 |
| 卷号 | 59期号:1页码:135-141 |
| 关键词 | Biomacromolecules Current amplitude Low concentrations Secondary structures signature event Single channel recording Small organic molecules translocation |
| 通讯作者 | 刘蕾（多） ; 吴海臣 |
| 英文摘要 | Nanopore has been developed to be a powerful, single-molecule analytical tool for sensing ions, small organic molecules and biomacromolecules such as proteins and DNAs. Generally, the identity of the analyte can be revealed by current amplitude changes and mean dwell time of the analyte binding events. In some cases, generation of highly characteristic current events affords an alternative way of analyte determination with high confidence level. However, we found that secondary structures in DNA/RNA hybrids might severely hinder the generation of signature events during their translocation through α-hemolysin nanopore. In this report, we propose a strategy to add a certain concentration of urea in the buffer solution for single channel recordings and validate that low concentration of urea can effectively denature the secondary structures in DNA hybrids and recover the generation of signature events. This finding might be useful in other secondary structure-related nanopore sensing activities. © 2015 Science China Press and Springer-Verlag Berlin Heidelberg |
| 类目[WOS] | 461.2 Biological Materials and Tissue Engineering ; 461.9 Biology ; 761 Nanotechnology ; 804.1 Organic Compounds ; 931.3 Atomic and Molecular Physics ; 933 Solid State Physics |
| 收录类别 | EI ; CA |
| 公开日期 | 2016-05-03 |
| 源URL | [http://ir.ihep.ac.cn/handle/311005/228916]  |
| 专题 | 高能物理研究所_多学科研究中心 高能物理研究所_核技术应用研究中心 |
| 推荐引用方式 GB/T 7714 | Wang,Xingyong,Li YR,et al. The effect of secondary structures on the generation of characteristic events during the translocation of DNA hybrid through α-hemolysin[J]. SCIENCE CHINA-CHEMISTRY,2015,59(1):135-141. |
| APA | Wang.,Xingyong.,李玉茹.,李婷.,刘蕾.,...&Hai-Chen.(2015).The effect of secondary structures on the generation of characteristic events during the translocation of DNA hybrid through α-hemolysin.SCIENCE CHINA-CHEMISTRY,59(1),135-141. |
| MLA | Wang,et al."The effect of secondary structures on the generation of characteristic events during the translocation of DNA hybrid through α-hemolysin".SCIENCE CHINA-CHEMISTRY 59.1(2015):135-141. |
入库方式： OAI收割
来源：高能物理研究所
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