Hairpin oligonucleotides anchored terbium ion: a fluorescent probe to specifically detect lead(II) at sub-nM levels
文献类型：期刊论文
| 作者 | Wei YT(魏月腾); Liu R(刘茹) ; Wang YL(王亚玲); Wei, YT; Liu, R; Wang, YL; Zhao, YL; Cai, ZF; Gao, XY; Zhao YL(赵宇亮)
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| 刊名 | ANALYST
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| 出版日期 | 2013 |
| 卷号 | 138期号:8页码:2302-2307 |
| 英文摘要 | A terbium based fluorescent probe was synthesized by coordinating terbium ions with a designed oligonucleotides (5'-ATATGGGGGATAT-3', termed GH5). GH5 improved the fluorescence of terbium ions by four orders of magnitude. The fluorescence enhancement of terbium ions by different oligonucleotides sequences indicated that the polyguanine loop of the hairpin GH5 is key to enhance terbium ion emission. The quantum yield of Tb-GH5 probe was 10.5% and the probe was photo-stable. The result of conductivity titration indicated that the stoichiometry of the probe is 3.5 Tb: 1 GH5, which is confirmed by fluorescence titration. This probe had high sensitivity and specificity for the detection of lead ions. The fluorescence intensity of this probe was linear with respect to lead concentration over a range 0.3-2.1 nM (R-2 = 0.99). The limit of detection for lead ions was 0.1 nM at a signal-to-noise ratio of 3. |
| 学科主题 | Chemistry |
| 收录类别 | SCI |
| WOS记录号 | WOS:000316222300012 |
| 公开日期 | 2016-05-03 |
| 源URL | [http://ir.ihep.ac.cn/handle/311005/224198]  |
| 专题 | 高能物理研究所_院士 高能物理研究所_多学科研究中心 |
| 推荐引用方式 GB/T 7714 | Wei YT,Liu R,Wang YL,et al. Hairpin oligonucleotides anchored terbium ion: a fluorescent probe to specifically detect lead(II) at sub-nM levels[J]. ANALYST,2013,138(8):2302-2307. |
| APA | 魏月腾.,刘茹.,王亚玲.,Wei, YT.,Liu, R.,...&高学云.(2013).Hairpin oligonucleotides anchored terbium ion: a fluorescent probe to specifically detect lead(II) at sub-nM levels.ANALYST,138(8),2302-2307. |
| MLA | 魏月腾,et al."Hairpin oligonucleotides anchored terbium ion: a fluorescent probe to specifically detect lead(II) at sub-nM levels".ANALYST 138.8(2013):2302-2307. |
入库方式： OAI收割
来源：高能物理研究所
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