Quantitative Analysis of Gold Nanoparticles in Single Cells by Laser Ablation Inductively Coupled Plasma-Mass Spectrometry
文献类型：期刊论文
| 作者 | Wang M(王萌) ; Zheng LN(郑丽娜); Wang B(汪冰); Wang, M; Zheng, LN; Wang, B; Chen, HQ; Zhao, YL; Chai, ZF; Reid, HJ
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| 刊名 | ANALYTICAL CHEMISTRY
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| 出版日期 | 2014 |
| 卷号 | 86期号:20页码:10252-10256 |
| 英文摘要 | Single cell analysis has become an important field of research in recent years reflecting the heterogeneity of cellular responses in biological systems. Here, we demonstrate a new method, based on laser ablation inductively coupled plasma mass spectrometry (LA-ICPMS), which can quantify in situ gold nanoparticles (Au NPs) in single cells. Dried residues of picoliter droplets ejected by a commercial inkjet printer were used to simulate matrix-matched calibration standards. The gold mass in single cells exposed to 100 nM NIST Au NPs (Reference material 8012, 30 nm) for 4 h showed a log-normal distribution, ranging from 1.7 to 72 fg Au per cell, which approximately corresponds to 9 to 370 Au NPs per cell. The average result from 70 single cells (15 +/- 13 fg Au per cell) was in good agreement with the result from an aqua regia digest solution of 1.2 x 10(6) cells (18 +/- 1 fg Au per cell). The limit of quantification was 1.7 fg Au. This paper demonstrates the great potential of LA-ICPMS for single cell analysis and the beneficial study of biological responses to metal drugs or NPs at the single cell level. |
| 学科主题 | Chemistry |
| 收录类别 | SCI |
| WOS记录号 | WOS:000343639800037 |
| 公开日期 | 2016-05-03 |
| 源URL | [http://ir.ihep.ac.cn/handle/311005/224810]  |
| 专题 | 高能物理研究所_院士 高能物理研究所_多学科研究中心 |
| 推荐引用方式 GB/T 7714 | Wang M,Zheng LN,Wang B,et al. Quantitative Analysis of Gold Nanoparticles in Single Cells by Laser Ablation Inductively Coupled Plasma-Mass Spectrometry[J]. ANALYTICAL CHEMISTRY,2014,86(20):10252-10256. |
| APA | 王萌.,郑丽娜.,汪冰.,Wang, M.,Zheng, LN.,...&丰伟悦.(2014).Quantitative Analysis of Gold Nanoparticles in Single Cells by Laser Ablation Inductively Coupled Plasma-Mass Spectrometry.ANALYTICAL CHEMISTRY,86(20),10252-10256. |
| MLA | 王萌,et al."Quantitative Analysis of Gold Nanoparticles in Single Cells by Laser Ablation Inductively Coupled Plasma-Mass Spectrometry".ANALYTICAL CHEMISTRY 86.20(2014):10252-10256. |
入库方式： OAI收割
来源：高能物理研究所
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