Quantitative analysis of iron in protein bands existing in polyacrylamide gel matrix with synchrotron radiation x-ray fluorescence
文献类型：期刊论文
| 作者 | Liu YB(刘颖斌); Gao YX(高愈希) ; Chen CY(陈春英); Li B(李柏); Yu HW(喻宏伟); Wang JX(王江雪); He W(何伟); Huang YY(黄宇营); Chai ZF(柴之芳); Liu, YB
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| 刊名 | HIGH ENERGY PHYSICS AND NUCLEAR PHYSICS-CHINESE EDITION
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| 出版日期 | 2005 |
| 卷号 | 29页码:#REF! |
| 关键词 | trace element speciation analysis synchrotron radiation X-ray fluorescence electrophoresis metalloprotein |
| 其他题名 | 聚丙烯酰胺凝胶基体中蛋白条带内金属含量的同步辐射X荧光定量分析 |
| 通讯作者 | Liu, YB (reprint author), CAS, Inst High Energy Phys, Beijing 100049, Peoples R China. |
| 英文摘要 | A procedure has been proposed for quantification of Fe in protein bands in polyacrylamide gel matrix with synchrotron radiation X-ray fluorescence(SRXRF) analysis. Human hemoglobin was separated with isoelectric focusing (IEF), and Fe amounts in protein bands were measured with SRXRF. Calibration standards were prepared by electrophoresis of bovine serum albumin containing known amounts of Fe with stacking gel. The calibration curves can be obtained in a range of 0-5.1ng Fe and linear relationship between dosage of metals and fluorescent intensity can be observed (R-2 = 0.9897). The method provides detection limit of 0.63ng Fe, and the recovery of Fe determination is about 106%. The hyphenated technique of SRXRF and IEF has advantages of quantifying trace elements in proteins and identifying the proteins simultaneously. It can be employed for speciation analysis of trace elements in biological samples. |
| 学科主题 | Physics |
| 类目[WOS] | Physics, Nuclear ; Physics, Particles & Fields |
| 收录类别 | SCI |
| WOS记录号 | WOS:000234206900016 |
| 公开日期 | 2016-05-03 |
| 源URL | [http://ir.ihep.ac.cn/handle/311005/225844]  |
| 专题 | 高能物理研究所_院士 高能物理研究所_实验物理中心 |
| 推荐引用方式 GB/T 7714 | Liu YB,Gao YX,Chen CY,et al. Quantitative analysis of iron in protein bands existing in polyacrylamide gel matrix with synchrotron radiation x-ray fluorescence[J]. HIGH ENERGY PHYSICS AND NUCLEAR PHYSICS-CHINESE EDITION,2005,29:#REF!. |
| APA | 刘颖斌.,高愈希.,陈春英.,李柏.,喻宏伟.,...&Chai, ZF.(2005).Quantitative analysis of iron in protein bands existing in polyacrylamide gel matrix with synchrotron radiation x-ray fluorescence.HIGH ENERGY PHYSICS AND NUCLEAR PHYSICS-CHINESE EDITION,29,#REF!. |
| MLA | 刘颖斌,et al."Quantitative analysis of iron in protein bands existing in polyacrylamide gel matrix with synchrotron radiation x-ray fluorescence".HIGH ENERGY PHYSICS AND NUCLEAR PHYSICS-CHINESE EDITION 29(2005):#REF!. |
入库方式： OAI收割
来源：高能物理研究所
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