Investigation of mercury-containing proteins by enriched stable isotopic tracer and size-exclusion chromatography hyphenated to inductively coupled plasma-isotope dilution mass spectrometry
文献类型：期刊论文
| ; | |
| 作者 | Shi JW(史俊稳); Feng WY(丰伟悦) ; Wang M(王萌); Zhang F(张芳); Li B(李柏); Wang B(汪冰); Zhu MT(朱墨桃); Chai ZF(柴之芳); Shi, JW; Feng, WY
|
| 刊名 | ANALYTICA CHIMICA ACTA
 ; ANALYTICA CHIMICA ACTA
|
| 出版日期 | 2007 ; 2007 |
| 卷号 | 583期号:1页码:#REF! |
| 关键词 | mercury-containing proteins stable isotopic tracer size-exclusion chromatography reverse isotope dilution ICP-MS mercury-containing proteins stable isotopic tracer size-exclusion chromatography reverse isotope dilution ICP-MS |
| DOI | 10.1016/j.aca.2006.09.040 |
| 通讯作者 | Feng, WY (reprint author), Chinese Acad Sci, Inst High Energy Phys, Lab Bioenvironm Hlth Sci Nanoscale Mat, Beijing 100049, Peoples R China. ; Feng, WY (reprint author), Chinese Acad Sci, Inst High Energy Phys, Lab Bioenvironm Hlth Sci Nanoscale Mat, Beijing 100049, Peoples R China. |
| 文献子类 | Article |
| 英文摘要 | In order to investigate trace mercury-containing proteins in maternal rat and their offspring, a method of enriched stable isotopic tracer (Hg-196 and Hg-198) combined with size-exclusion chromatography (SEC) coupled to inductively coupled plasma-isotope dilution mass spectrometry (ICP-IDMS) was developed. Prior to the analysis, Hg-196- and Hg-198-enriched methylmercury was administrated to the pregnant rats. Then the mercury-containing proteins in serum and brain cytosol of the dam and pup rats were separated by size-exclusion columns and the mercury was detected by ICP-MS. The ICP-MS spectrogram of the tracing samples showed significantly elevated Hg-196 and Hg-198 isotopic signals compared with the natural ones, indicating that the detection sensitivity could be increased by the tracer method. The contents of mercury in chromatographic fractions of the dam and pup rat brain cytosol were quantitatively estimated by post-column reverse ID-ICP-MS. The quantitative speciation differences of mercury in brain cytosol between the dam and pup rats were observed, indicating that such studies could be useful for toxicological estimation. Additionally, the isotopic ratio measurement of Hg-198/Hg-202 in the tracing samples could be used to identify the artifact mercury species caused in the analytical procedure. The study demonstrates that the tracer method combined with high-performance liquid chromatography (HPLC)-ICP-IDMS could provide reliably qualitative and quantitative information on mercury-containing proteins in organisms. (c) 2006 Elsevier B.V. All rights reserved.; In order to investigate trace mercury-containing proteins in maternal rat and their offspring, a method of enriched stable isotopic tracer (Hg-196 and Hg-198) combined with size-exclusion chromatography (SEC) coupled to inductively coupled plasma-isotope dilution mass spectrometry (ICP-IDMS) was developed. Prior to the analysis, Hg-196- and Hg-198-enriched methylmercury was administrated to the pregnant rats. Then the mercury-containing proteins in serum and brain cytosol of the dam and pup rats were separated by size-exclusion columns and the mercury was detected by ICP-MS. The ICP-MS spectrogram of the tracing samples showed significantly elevated Hg-196 and Hg-198 isotopic signals compared with the natural ones, indicating that the detection sensitivity could be increased by the tracer method. The contents of mercury in chromatographic fractions of the dam and pup rat brain cytosol were quantitatively estimated by post-column reverse ID-ICP-MS. The quantitative speciation differences of mercury in brain cytosol between the dam and pup rats were observed, indicating that such studies could be useful for toxicological estimation. Additionally, the isotopic ratio measurement of Hg-198/Hg-202 in the tracing samples could be used to identify the artifact mercury species caused in the analytical procedure. The study demonstrates that the tracer method combined with high-performance liquid chromatography (HPLC)-ICP-IDMS could provide reliably qualitative and quantitative information on mercury-containing proteins in organisms. (c) 2006 Elsevier B.V. All rights reserved. |
| 学科主题 | Chemistry ; Chemistry |
| 类目[WOS] | Chemistry, Analytical |
| 收录类别 | SCI |
| WOS记录号 | WOS:000243705500012 ; WOS:000243705500012 |
| 公开日期 | 2016-05-03 |
| 源URL | [http://ir.ihep.ac.cn/handle/311005/226025]  |
| 专题 | 高能物理研究所_院士 高能物理研究所_实验物理中心 高能物理研究所_多学科研究中心 |
| 推荐引用方式 GB/T 7714 | Shi JW,Feng WY,Wang M,et al. Investigation of mercury-containing proteins by enriched stable isotopic tracer and size-exclusion chromatography hyphenated to inductively coupled plasma-isotope dilution mass spectrometry, Investigation of mercury-containing proteins by enriched stable isotopic tracer and size-exclusion chromatography hyphenated to inductively coupled plasma-isotope dilution mass spectrometry[J]. ANALYTICA CHIMICA ACTA, ANALYTICA CHIMICA ACTA,2007, 2007,583, 583(1):#REF!, #REF!. |
| APA | 史俊稳.,丰伟悦.,王萌.,张芳.,李柏.,...&Chai, ZF.(2007).Investigation of mercury-containing proteins by enriched stable isotopic tracer and size-exclusion chromatography hyphenated to inductively coupled plasma-isotope dilution mass spectrometry.ANALYTICA CHIMICA ACTA,583(1),#REF!. |
| MLA | 史俊稳,et al."Investigation of mercury-containing proteins by enriched stable isotopic tracer and size-exclusion chromatography hyphenated to inductively coupled plasma-isotope dilution mass spectrometry".ANALYTICA CHIMICA ACTA 583.1(2007):#REF!. |
入库方式： OAI收割
来源：高能物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。