Investigation of zinc-containing peptide deformylase from Leptospira interrogans by X-ray absorption near-edge spectroscopy
文献类型：期刊论文
| 作者 | Li SJ(李树军); Chu WS(储旺盛) ; Hu TD(胡天斗); Xie YN(谢亚宁); Xian DC(冼鼎昌); Wu ZY(吴自玉); Li, SJ; Zhou, ZC; Chu, WS; Gong, WM
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| 刊名 | JOURNAL OF SYNCHROTRON RADIATION
 |
| 出版日期 | 2005 |
| 卷号 | 12期号:1页码:#REF! |
| 关键词 | peptide deformylase metalloproteinases catalytic mechanism BioXAS |
| 通讯作者 | Gong, WM (reprint author), Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Beijing 100101, Peoples R China. |
| 英文摘要 | Peptide deformylase (PDF, EC 3.5.1.27) is essential for the normal growth of eubacterium but not for mammalians. Recently, PDF has been studied as a target for new antibiotics. Its activity is strongly dependent on the bound metal ion. The crystallographic studies did not show any significant structural difference upon various bound metal ions. In this paper, X-ray absorption spectroscopy was employed to determine the local structure around the zinc ion of PDF from Leptospira interrogans in dry powder. XANES (X-ray absorption near-edge structure) calculations were performed and the local geometry of the active center was reconstructed successfully. By comparing with the crystal structure of an enzyme-product complex, the results from calculations show that a water molecule has moved towards the zinc ion and lies in the distance range to coordinate with the zinc ion weakly. |
| 学科主题 | Instruments & Instrumentation; Optics; Physics |
| 类目[WOS] | Instruments & Instrumentation ; Optics ; Physics, Applied |
| 收录类别 | SCI |
| WOS记录号 | WOS:000225960900019 |
| 公开日期 | 2016-05-03 |
| 源URL | [http://ir.ihep.ac.cn/handle/311005/226230]  |
| 专题 | 高能物理研究所_院士 高能物理研究所_多学科研究中心 |
| 推荐引用方式 GB/T 7714 | Li SJ,Chu WS,Hu TD,et al. Investigation of zinc-containing peptide deformylase from Leptospira interrogans by X-ray absorption near-edge spectroscopy[J]. JOURNAL OF SYNCHROTRON RADIATION,2005,12(1):#REF!. |
| APA | 李树军.,储旺盛.,胡天斗.,谢亚宁.,冼鼎昌.,...&Wu, ZY.(2005).Investigation of zinc-containing peptide deformylase from Leptospira interrogans by X-ray absorption near-edge spectroscopy.JOURNAL OF SYNCHROTRON RADIATION,12(1),#REF!. |
| MLA | 李树军,et al."Investigation of zinc-containing peptide deformylase from Leptospira interrogans by X-ray absorption near-edge spectroscopy".JOURNAL OF SYNCHROTRON RADIATION 12.1(2005):#REF!. |
入库方式： OAI收割
来源：高能物理研究所
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