Facile Approach To Observe and Quantify the alpha(IIb)beta(3) Integrin on a Single-Cell
文献类型：期刊论文
| 作者 | Zhai J(翟蛟) ; Wang YL(王亚玲); Xu C(徐超); Zheng LN(郑令娜); Wang M(王萌); Feng WY(丰伟悦); Gao J(高靓); Zhao LN(赵丽娜); Liu R(刘茹); Gao FP(高福平)
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| 刊名 | ANALYTICAL CHEMISTRY
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| 出版日期 | 2015 |
| 卷号 | 87期号:5页码:2546-2549 |
| 通讯作者 | 王亚玲 ; 高学云 |
| 英文摘要 | We report for the first time seeing and counting integrin a(IIb)beta(3) on a single-cell level. The proposed method is based on the using of the Au cluster probe. With the fluorescent property of Au-24 cluster and the specific targeting ability of peptide, our probe can directly visualize integrin a(IIb)beta(3) on the membrane of human erythroleukemia cells (HEL) via confocal microscopy. On the basis of the accurate formula of our probe (Au(24)Peptide(8)), the number of integrin a(IIb)beta(3) can be precisely counted by quantifying the gold content on a single HEL cell via laser ablation inductively coupled plasma mass spectrometry. Our results reveal that the number of integrin a(IIb)beta(3) on a single cell varies from 5.75 x 10(-17) to 9.11 x 10(-17) mol, because of the heteroexpression levels of a(IIb)beta(3) on individual cells. |
| 学科主题 | Chemistry |
| 类目[WOS] | Chemistry, Analytical |
| 收录类别 | SCI ; EI ; CA |
| WOS记录号 | WOS:000350611700006 |
| 公开日期 | 2016-05-03 |
| 源URL | [http://ir.ihep.ac.cn/handle/311005/228242]  |
| 专题 | 高能物理研究所_院士 高能物理研究所_多学科研究中心 |
| 推荐引用方式 GB/T 7714 | Zhai J,Wang YL,Xu C,et al. Facile Approach To Observe and Quantify the alpha(IIb)beta(3) Integrin on a Single-Cell[J]. ANALYTICAL CHEMISTRY,2015,87(5):2546-2549. |
| APA | 翟蛟.,王亚玲.,徐超.,郑令娜.,王萌.,...&Gao, XY.(2015).Facile Approach To Observe and Quantify the alpha(IIb)beta(3) Integrin on a Single-Cell.ANALYTICAL CHEMISTRY,87(5),2546-2549. |
| MLA | 翟蛟,et al."Facile Approach To Observe and Quantify the alpha(IIb)beta(3) Integrin on a Single-Cell".ANALYTICAL CHEMISTRY 87.5(2015):2546-2549. |
入库方式： OAI收割
来源：高能物理研究所
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