Peptide-Conjugated Gold Nanoprobe: Intrinsic Nanozyme-Linked Immunosorbent Assay of Integrin Expression Level on Cell Membrane.
文献类型：期刊论文
| 作者 | Gao J(高靓) ; Liu MQ(刘梅青); Ma GF(马桂服); Wang YL(王亚玲); Zhao LN(赵丽娜); Yuan Q(袁卿); Gao FP(高福平); Liu R(刘茹); Zhai J(翟皎); Chai ZF(柴之芳)
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| 刊名 | ACS NANO
 |
| 出版日期 | 2015 |
| 卷号 | 9期号:11页码:10979-10990 |
| 关键词 | nanozyme enzyme-linked immunosorbent assay gold nanoparticles membrane protein quantification two-photon imaging |
| 通讯作者 | 高学云 |
| 英文摘要 | Precisely quantifying the membrane protein expression level on cell surfaces is of vital importance for early cancer diagnosis and efficient treatment. We demonstrate that gold nanoparticle bioconjugated by a rationally designed peptide as nanoprobe possesses selective labeling and accurate quantification capacity of integrin GPIlb/IIIa on the human erythroleukemia cell line. Through selective recognition and marking of integrin, two-photon photoluminescence of the nanoprobe is exploited for direct observation of protein spatial distribution on cell membrane. More importantly, utilizing intrinsic enzyme-like catalysis property of the nanoprobe, the expression level of integrin on human erythroleukemia cells can be quantitatively counted in an amplified and reliable colorimetric assay without cell lysis and protein extraction process. In addition, the analysis of the correlation between the gold nanoparticle and the membrane protein via relevant inductively coupled plasma mass spectrometry measurement verifies the reliability of the new analytical method. It is anticipated that this facile and efficient strategy holds a great promise for a rapid, precise, and reliable quantification of interested functional membrane proteins on the cell surface. |
| 学科主题 | Chemistry; Science & Technology - Other Topics; Materials Science |
| 类目[WOS] | Chemistry, Multidisciplinary ; Chemistry, Physical ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary |
| 收录类别 | SCI ; EI ; CA |
| WOS记录号 | WOS:000365464800046 |
| 公开日期 | 2016-05-03 |
| 源URL | [http://ir.ihep.ac.cn/handle/311005/228877]  |
| 专题 | 高能物理研究所_院士 高能物理研究所_多学科研究中心 |
| 推荐引用方式 GB/T 7714 | Gao J,Liu MQ,Ma GF,et al. Peptide-Conjugated Gold Nanoprobe: Intrinsic Nanozyme-Linked Immunosorbent Assay of Integrin Expression Level on Cell Membrane.[J]. ACS NANO,2015,9(11):10979-10990. |
| APA | 高靓.,刘梅青.,马桂服.,王亚玲.,赵丽娜.,...&Xueyun..(2015).Peptide-Conjugated Gold Nanoprobe: Intrinsic Nanozyme-Linked Immunosorbent Assay of Integrin Expression Level on Cell Membrane..ACS NANO,9(11),10979-10990. |
| MLA | 高靓,et al."Peptide-Conjugated Gold Nanoprobe: Intrinsic Nanozyme-Linked Immunosorbent Assay of Integrin Expression Level on Cell Membrane.".ACS NANO 9.11(2015):10979-10990. |
入库方式： OAI收割
来源：高能物理研究所
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