Mapping the Distribution of Cysts from the Toxic Dinoflagellate Cochlodinium polykrikoides in Bloom-Prone Estuaries by a Novel Fluorescence In Situ Hybridization Assay
文献类型：期刊论文
| 作者 | Hattenrath-Lehmann, Theresa K.1; Zhen, Yu2,3; Wallace, Ryan B.1; Tang, Ying-Zhong4; Gobler, Christopher J.1 |
| 刊名 | APPLIED AND ENVIRONMENTAL MICROBIOLOGY
 |
| 出版日期 | 2016-02-01 |
| 卷号 | 82期号:4页码:1114-1125 |
| 英文摘要 | Cochlodinium polykrikoides is a cosmopolitan dinoflagellate that is notorious for causing fish-killing harmful algal blooms (HABs) across North America and Asia. While recent laboratory and ecosystem studies have definitively demonstrated that Cochlodinium forms resting cysts that may play a key role in the dynamics of its HABs, uncertainties regarding cyst morphology and detection have prohibited even a rudimentary understanding of the distribution of C. polykrikoides cysts in coastal ecosystems. Here, we report on the development of a fluorescence in situ hybridization (FISH) assay using oligonucleotide probes specific for the large subunit (LSU) ribosomal DNA (rDNA) of C. polykrikoides. The LSU rDNA-targeted FISH assay was used with epifluorescence microscopy and was iteratively refined to maximize the fluorescent reaction with C. polykrikoides and minimize cross-reactivity. The final LSU rDNA-targeted FISH assay was found to quantitatively recover cysts made by North American isolates of C. polykrikoides but not cysts formed by other common cyst-forming dinoflagellates. The method was then applied to identify and map C. polykrikoides cysts across bloom-prone estuaries. Annual cyst and vegetative cell surveys revealed that elevated densities of C. polykrikoides cysts (> 100 cm(-3)) during the spring of a given year were spatially consistent with regions of dense blooms the prior summer. The identity of cysts in sediments was confirmed via independent amplification of C. polykrikoides rDNA. This study mapped C. polykrikoides cysts in a natural marine setting and indicates that the excystment of cysts formed by this harmful alga may play a key role in the development of HABs of this species. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000369375900014 |
| 公开日期 | 2016-05-03 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/112446]  |
| 专题 | 海洋研究所_海洋生态与环境科学重点实验室 |
| 作者单位 | 1.SUNY Stony Brook, Sch Marine & Atmospher Sci, Southampton, NY USA 2.Ocean Univ China, Coll Environm Sci & Engn, Qingdao, Peoples R China 3.Ocean Univ China, Minist Educ, Key Lab Marine Environm & Ecol, Qingdao, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hattenrath-Lehmann, Theresa K.,Zhen, Yu,Wallace, Ryan B.,et al. Mapping the Distribution of Cysts from the Toxic Dinoflagellate Cochlodinium polykrikoides in Bloom-Prone Estuaries by a Novel Fluorescence In Situ Hybridization Assay[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2016,82(4):1114-1125. |
| APA | Hattenrath-Lehmann, Theresa K.,Zhen, Yu,Wallace, Ryan B.,Tang, Ying-Zhong,&Gobler, Christopher J..(2016).Mapping the Distribution of Cysts from the Toxic Dinoflagellate Cochlodinium polykrikoides in Bloom-Prone Estuaries by a Novel Fluorescence In Situ Hybridization Assay.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,82(4),1114-1125. |
| MLA | Hattenrath-Lehmann, Theresa K.,et al."Mapping the Distribution of Cysts from the Toxic Dinoflagellate Cochlodinium polykrikoides in Bloom-Prone Estuaries by a Novel Fluorescence In Situ Hybridization Assay".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 82.4(2016):1114-1125. |
入库方式： OAI收割
来源：海洋研究所
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