Stomatin-like protein 2 of turbot Scopthalmus maximus: Gene cloning, expression profiling and immunoregulatory properties
文献类型：期刊论文
| 作者 | Chi, Heng1,2; Hu, Yong-hua1,2 |
| 刊名 | FISH & SHELLFISH IMMUNOLOGY
 |
| 出版日期 | 2016-02-01 |
| 卷号 | 49页码:436-441 |
| 关键词 | Stomatin like protein 2 Scopthalmus maximus Gene cloning Immunoregulation |
| 英文摘要 | Stomatin-like protein 2 (SLP-2) is a novel and unusual member of the stomatin gene superfamily. In this study, we obtained a full-length SLP-2 (SmSLP-2) cDNA from turbot (Scopthalmus maximus) spleen cDNA library. The cDNA sequence of SmSLP-2 contains a 5'-UTR of 107 bp, an ORF of 1050 bp, and a 3'-UTR of 959 bp. The ORF encodes a putative protein of 349 residues, which has a calculated molecular mass of 38.7 kDa. The SmSLP-2 protein possesses a prohibitin-homology (PHB) domain (residues 40 to 198) and shares 72.4-87.6% overall sequence identity with that of the teleost species. The highest expression of SmSLP-2 mRNA was found in the skin, followed by the head kidney, gut, spleen, liver, heart, gill and muscle. Moreover, both viral and bacterial pathogen infection resulted in the up-regulation of SmSLP-2 mRNA in the turbot head kidney and spleen in vivo. Subcellular localization analysis indicated that the SmSLP-2 proteins are mainly located in the peripheral membrane of ZF4 cells. This study also demonstrated that SmSLP-2 modulates IL-2 expression via active NF kappa B signaling pathway, and is possibly involved in host immune defense against bacterial and viral pathogens. (C) 2016 Elsevier Ltd. All rights reserved. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000370884200047 |
| 公开日期 | 2016-05-03 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/112476]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chi, Heng,Hu, Yong-hua. Stomatin-like protein 2 of turbot Scopthalmus maximus: Gene cloning, expression profiling and immunoregulatory properties[J]. FISH & SHELLFISH IMMUNOLOGY,2016,49:436-441. |
| APA | Chi, Heng,&Hu, Yong-hua.(2016).Stomatin-like protein 2 of turbot Scopthalmus maximus: Gene cloning, expression profiling and immunoregulatory properties.FISH & SHELLFISH IMMUNOLOGY,49,436-441. |
| MLA | Chi, Heng,et al."Stomatin-like protein 2 of turbot Scopthalmus maximus: Gene cloning, expression profiling and immunoregulatory properties".FISH & SHELLFISH IMMUNOLOGY 49(2016):436-441. |
入库方式： OAI收割
来源：海洋研究所
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