Enhancing DNA Detection Sensitivity through a Two-Step Enrichment Method with Magnetic Beads and Droplet Evaporation
文献类型：期刊论文
| 作者 | Li JO (李炯)
|
| 刊名 | Analytical Letters
 |
| 出版日期 | 2010-09 |
| 卷号 | 43期号:9页码:1525-1533 |
| 关键词 | DNA detection sensitivity Droplet evaporation Magnetic beads |
| 通讯作者 | Li JO (李炯) |
| 英文摘要 | A simple and sensitive DNA detection method has been developed through a two-step enrichment process. Trace amount of target DNA in a large volume (1 mL) is selectively separated and condensed with DNA-modified magnetic beads into a small volume (5 mu L) by denaturalization. Then, the pre-enriched target DNA solution (1 mu L) is transferred onto a smooth hydrophobic surface, where the target DNA is further-enriched by natural sessile droplet evaporation. Using this method, the fluorescence detection sensitivity of the target DNA can be enhanced by 3 orders of magnitude and as low as 3.91pM of the target DNA can be detected within 2 hours. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000278775700010 |
| 公开日期 | 2010-12-31 |
| 源URL | [http://58.210.77.100/handle/332007/283]  |
| 专题 | 苏州纳米技术与纳米仿生研究所_纳米生化平台 |
| 通讯作者 | Li JO (李炯) |
| 推荐引用方式 GB/T 7714 | Li JO . Enhancing DNA Detection Sensitivity through a Two-Step Enrichment Method with Magnetic Beads and Droplet Evaporation[J]. Analytical Letters,2010,43(9):1525-1533. |
| APA | Li JO .(2010).Enhancing DNA Detection Sensitivity through a Two-Step Enrichment Method with Magnetic Beads and Droplet Evaporation.Analytical Letters,43(9),1525-1533. |
| MLA | Li JO ."Enhancing DNA Detection Sensitivity through a Two-Step Enrichment Method with Magnetic Beads and Droplet Evaporation".Analytical Letters 43.9(2010):1525-1533. |
入库方式： OAI收割
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。