Polysaccharide-coated beads platform for biomolecule analysis Evolution of SiO2-based suspension arrays
文献类型：期刊论文
| 作者 | Li JO (李炯) ; Zheng KX (郑克孝); Jiang L (姜丽)
|
| 刊名 | Carbohydrate Polymers
 |
| 出版日期 | 2011-01-10 |
| 卷号 | 83期号:2页码:818-823 |
| 关键词 | Suspension array Polysaccharide Protein immobilization Silica beads |
| 通讯作者 | Li JO (李炯) |
| 合作状况 | 其它 |
| 英文摘要 | Graphically encoded SiO2-based suspension arrays are often manufactured with the 2D matrixes by popular aldehyde-aminosilane chemistry for the attachment of a variety of biomolecules including peptides proteins nucleic acid probes etc In view of the relatively low immobilization efficiency we have developed a novel method to prepare polysaccharide-coated silica beads which can increase the binding capacity of biomolecules on silica beads and improve the performance of the stability of biomolecules under various denaturing conditions In this study two kinds of representative polysaccharides chitosan and sodium alginate (NaAlg) were used for the surface coating And two procedures for the immobilization of protein on NaAlg-coated beads were also studied Under the optimized experimental conditions NaAlg-coated beads had excellent biomolecular binding capacities which increased the fluorescence signal by almost 80% Attending to the results obtained we believe that the proposed methods may have significant potential for novel SiO2-based suspension array technology. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000285437000069 |
| 公开日期 | 2011-03-11 |
| 源URL | [http://58.210.77.100/handle/332007/319]  |
| 专题 | 苏州纳米技术与纳米仿生研究所_纳米生化平台 |
| 通讯作者 | Li JO (李炯) |
| 推荐引用方式 GB/T 7714 | Li JO ,Zheng KX ,Jiang L . Polysaccharide-coated beads platform for biomolecule analysis Evolution of SiO2-based suspension arrays[J]. Carbohydrate Polymers,2011,83(2):818-823. |
| APA | Li JO ,Zheng KX ,&Jiang L .(2011).Polysaccharide-coated beads platform for biomolecule analysis Evolution of SiO2-based suspension arrays.Carbohydrate Polymers,83(2),818-823. |
| MLA | Li JO ,et al."Polysaccharide-coated beads platform for biomolecule analysis Evolution of SiO2-based suspension arrays".Carbohydrate Polymers 83.2(2011):818-823. |
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