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The promotion of neurite sprouting and outgrowth of mouse hippocampal cells in culture by graphene substrates
文献类型：期刊论文
| 作者 | Liu, LW (刘立伟) ; Song, Q (宋琴); Kong, T (孔涛); Su, RG (苏瑞巩); Cheng, GS (程国胜)
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| 刊名 | BIOMATERIALS
 |
| 出版日期 | 2011-12 |
| 卷号 | 32期号:35页码:9374-9382 |
| 关键词 | Neuron-favorable CVD graphene GAP-43 Neurite sprouting & outgrowth Hippocampus |
| 通讯作者 | Tang, ML (唐明亮) |
| 英文摘要 | Graphene has been demonstrated in many biomedical applications and its potentials for neural interfacing. Emerging concerns on graphene, as a biomedical material, are its biocompatibility and how biologically targeted tissue/cells respond to it. Relatively few studies attempted to address the interactions of graphene or its derivatives with the tissues/cells, while very few reports on neural system. In this study, we tried to explore how neurites, one of the key structures for neural functions, are affected by graphene during the development until maturation in a mouse hippocampal culture model. The results reveal that graphene substrates exhibited excellent biocompatibility, as cell viability and morphology were not affected. Meanwhile, neurite numbers and average neurite length on graphene were significantly enhanced during 2-7 days after cell seeding compared with tissue culture polystyrene (TCPS) substrates. Especially on Day 2 of the neural development period, graphene substrates efficiently promoted neurite sprouting and outgrowth to the maximal extent. Additionally, expression of growth-associate protein-43 (GAP-43) was examined in both graphene and TCPS groups. Western blot analysis showed that GAP-43 expression was greatly enhanced in graphene group compared to TCPS group, which might result in the boost of neurite sprouting and outgrowth. This study suggests the potential of graphene as a material for neural interfacing and provides insight into the future biomedical applications of graphene. |
| 收录类别 | SCI ; EI |
| 语种 | 英语 |
| WOS记录号 | WOS:000296684200023 |
| 公开日期 | 2012-07-25 |
| 源URL | [http://58.210.77.100/handle/332007/520]  |
| 专题 | 苏州纳米技术与纳米仿生研究所_纳米生物医学与安全研究部_程国胜团队 |
| 推荐引用方式 GB/T 7714 | Liu, LW ,Song, Q ,Kong, T ,et al. The promotion of neurite sprouting and outgrowth of mouse hippocampal cells in culture by graphene substrates[J]. BIOMATERIALS,2011,32(35):9374-9382. |
| APA | Liu, LW ,Song, Q ,Kong, T ,Su, RG ,&Cheng, GS .(2011).The promotion of neurite sprouting and outgrowth of mouse hippocampal cells in culture by graphene substrates.BIOMATERIALS,32(35),9374-9382. |
| MLA | Liu, LW ,et al."The promotion of neurite sprouting and outgrowth of mouse hippocampal cells in culture by graphene substrates".BIOMATERIALS 32.35(2011):9374-9382. |
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