Serum tumor marker detection on PEGylated lipid membrane using biosensor based on total internal reflection imaging ellipsometry
文献类型：期刊论文
| 作者 | Chen, YY (陈艳艳) ; Chen, YY (陈艳艳)
|
| 刊名 | SENSORS AND ACTUATORS B-CHEMICAL
 |
| 出版日期 | 2011-11-28 |
| 卷号 | 159期号:1页码:121-125 |
| 关键词 | PEGylated phospholipid membrane Total internal reflection imaging ellipsometry CA19-9 |
| 通讯作者 | Chen, YY (陈艳艳) |
| 英文摘要 | We describe highly sensitive, label-free, real time detection of the biomarker CA19-9 using microarray biosensors based on total internal reflection imaging ellipsometry (TIRIE), in which anti-CA19-9 is immobilized onto the PEGylated phospholipid membrane. Strong resistance against non-specific adsorption of PEGylated lipid membrane allows direct serum assay without the blocking agent. We find that PEG density has an influence on antibody assembly and only a proper amount of PEG is appropriate for antigen binding. Two different methods for antibody assembly are compared and finally protein A is preferred due to its higher protein binding efficiency. The estimated detection limit of CA19-9 is 18.2 U/ml, which is lower than the cut-off value for normal level (37 U/ml). Regression analysis between the results of microarray biosensor and electrochemiluminescence immunoassay (ECLIA) suggests a high correlation (R = 0.989). The capability for real-time monitoring of tumor marker with high sensitivity and selectivity in clinically relevant samples opens up substantial possibilities for early cancer diagnosis and treatment. |
| 收录类别 | SCI ; EI |
| 语种 | 英语 |
| WOS记录号 | WOS:000295745900017 |
| 公开日期 | 2012-07-25 |
| 源URL | [http://58.210.77.100/handle/332007/519]  |
| 专题 | 苏州纳米技术与纳米仿生研究所_纳米生物医学与安全研究部_戴建武团队 |
| 通讯作者 | Chen, YY (陈艳艳); Chen, YY (陈艳艳) |
| 推荐引用方式 GB/T 7714 | Chen, YY ,Chen, YY . Serum tumor marker detection on PEGylated lipid membrane using biosensor based on total internal reflection imaging ellipsometry[J]. SENSORS AND ACTUATORS B-CHEMICAL,2011,159(1):121-125. |
| APA | Chen, YY ,&Chen, YY .(2011).Serum tumor marker detection on PEGylated lipid membrane using biosensor based on total internal reflection imaging ellipsometry.SENSORS AND ACTUATORS B-CHEMICAL,159(1),121-125. |
| MLA | Chen, YY ,et al."Serum tumor marker detection on PEGylated lipid membrane using biosensor based on total internal reflection imaging ellipsometry".SENSORS AND ACTUATORS B-CHEMICAL 159.1(2011):121-125. |
入库方式： OAI收割
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。