Detection of duck hepatitis virus serotype1 by biosensor based on imaging ellipsometry
文献类型：期刊论文
| 作者 | Chen, YY (陈艳艳)
|
| 刊名 | CURRENT APPLIED PHYSICS
 |
| 出版日期 | 2011-05 |
| 卷号 | 11期号:3页码:353-357 |
| 关键词 | Biosensor Imaging ellipsometry Duck hepatitis virus Detection |
| 英文摘要 | A novel method for the detection of duck hepatitis virus serotype1 (DHV1) using a biosensor based on imaging ellipsometry (IE) is developed. Polyclonal antibodies against DHV1 were immobilized on silicon wafers through protein A and used to capture virus particles in sample. Signals on silicon surface were acquired directly in grayscale format on an imaging ellipsometry image. This study demonstrated that the assay is rapid and accurate for detection of duck hepatitis virus. Compared with traditional methods, this technique not only has high sensitivity as low as 8 x 10(-9.5) LD(50)/ml, but can also simultaneously perform multiple serotype identification of DHV1, DHV1-g and DHV1-s. These results suggest that this biosensor might be a practicable diagnostic tool for duck hepatitis virus detection. |
| 收录类别 | SCI ; EI |
| 语种 | 英语 |
| WOS记录号 | WOS:000288183300017 |
| 公开日期 | 2012-08-24 |
| 源URL | [http://58.210.77.100/handle/332007/678]  |
| 专题 | 苏州纳米技术与纳米仿生研究所_纳米生物医学与安全研究部_戴建武团队 |
| 推荐引用方式 GB/T 7714 | Chen, YY . Detection of duck hepatitis virus serotype1 by biosensor based on imaging ellipsometry[J]. CURRENT APPLIED PHYSICS,2011,11(3):353-357. |
| APA | Chen, YY .(2011).Detection of duck hepatitis virus serotype1 by biosensor based on imaging ellipsometry.CURRENT APPLIED PHYSICS,11(3),353-357. |
| MLA | Chen, YY ."Detection of duck hepatitis virus serotype1 by biosensor based on imaging ellipsometry".CURRENT APPLIED PHYSICS 11.3(2011):353-357. |
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