中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Effects of simvastatin-loaded polymeric micelles on human osteoblast-like MG-63 cells

文献类型:期刊论文

作者Qiangbin Wang(王强斌); Jianwu Dai(戴建武)
刊名Colloids and Surfaces B: Biointerfaces
出版日期2012-08
卷号102期号:1页码:420-427
关键词Micelle Simvastatin Delivery system Osteoblast Bone regeneration
通讯作者Lei Zhou
英文摘要

To develop an optimized simvastatin (SV) delivery device for bone regeneration, SV-loaded poly(ethylene glycol)-poly(ɛ-caprolactone) (PECL) micelles were constructed. The micelles had an average size of 80 nm. The in vitro release behavior of SV from the micelles showed prolonged release compared to the free SV. The following four groups were tested in a cytologic experiment: a free SV group, a SV-loaded micelle group with SV concentrations ranging from 2.5 × 10−6 to 2.5 × 10−10 M, a drug-free micelle group and a blank control group. The effect of SV-loaded micelles on osteoblast-like MG-63 cells was determined via analysis of cell proliferation, alkaline phosphatase activity, and cell calcification. In addition, the mRNA and protein expression of the BMP-2 gene were determined with real-time fluorescence quantitative polymerase chain reaction and western blot techniques, respectively. The results show that SV-loaded PECL micelles cause effective suppression of the osteoblast early proliferation inhibition, stimulation of osteoblast differentiation and mineralization, and stimulation of the BMP-2 expression. Therefore, SV-loaded PECL micelles are predicted to have great potential in bone regeneration applications.


语种英语
WOS记录号WOS:000313301200061
公开日期2013-01-22
源URL[http://58.210.77.100/handle/332007/982]  
专题苏州纳米技术与纳米仿生研究所_纳米生物医学与安全研究部_戴建武团队
苏州纳米技术与纳米仿生研究所_纳米生物医学与安全研究部_王强斌团队
推荐引用方式
GB/T 7714
Qiangbin Wang,Jianwu Dai. Effects of simvastatin-loaded polymeric micelles on human osteoblast-like MG-63 cells[J]. Colloids and Surfaces B: Biointerfaces,2012,102(1):420-427.
APA Qiangbin Wang,&Jianwu Dai.(2012).Effects of simvastatin-loaded polymeric micelles on human osteoblast-like MG-63 cells.Colloids and Surfaces B: Biointerfaces,102(1),420-427.
MLA Qiangbin Wang,et al."Effects of simvastatin-loaded polymeric micelles on human osteoblast-like MG-63 cells".Colloids and Surfaces B: Biointerfaces 102.1(2012):420-427.

入库方式: OAI收割

来源:苏州纳米技术与纳米仿生研究所

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