In Vitro Hemocompatibility and Toxic Mechanism of Graphene Oxide on Human Peripheral Blood T Lymphocytes and Serum Albumin
文献类型：期刊论文
| 作者 | Ma, HW (马宏伟) ; Chen, YY (陈艳艳); Zhang, ZJ (张智军)
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| 刊名 | ACS APPLIED MATERIALS & INTERFACES
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| 出版日期 | 2014 |
| 卷号 | 6期号:22页码:19797-19807 |
| 关键词 | hemocompatibility graphene oxide plasma proteins T lymphocytes toxic mechanism |
| 通讯作者 | Chen, YY (陈艳艳) |
| 英文摘要 | Graphene oxide (GO) has shown tremendous application potential as a biomedical material. However, its interactions with blood components are not yet well understood. In this work, we assess the toxicity of pristine GO (p-GO) and functionalized GO (GO-COOH and GO-PEI) to primary human peripheral blood T lymphocytes and human serum albumin (HSA), and also study the underlying toxic mechanism. Our results indicate that p-GO and GO-COOH have good biocompatibility to T lymphocytes at the concentration below 25 mu g mL(-1), but notable cytotoxicity above 50 mu g mL(-1). By contrast, GO-PEI exhibits significant toxicity even at 1.6 mu g mL(-1). Further investigations show that although p-GO does not enter into the cell or damage the membrane, its presence leads to the increase in reactive oxygen species (ROS), moderate DNA damage, and T lymphocyte apoptosis. Interestingly, little effect on T lymphocyte immune response suppression is observed in this process despite p-GO inflicting cell apoptosis. The toxic mechanism is that p-GO interacts directly with the protein receptors to inhibit their ligand-binding ability, leading to ROS-dependent passive apoptosis through the B-cell lymphoma-2 (Bcl-2) pathway. Compared with p-GO, GO-COOH exhibits a similar toxic effect on T lymphocytes except keeping a normal ROS level. A proposed toxic mechanism is that GO-COOH inhibits protein receptor-ligand binding, and passes the passive apoptosis signal to nucleus DNA through a ROS-independent mechanism. On the other hand, GO-PEI shows severe hematotoxicity to T lymphocytes by inducing membrane damage. For plasma protein HSA, the binding of GO-COOH results in minimal conformational change and HSA's binding capacity to bilirubin remains unaffected, while the binding of p-GO and GO-PEI exhibits strong toxicity on HSA. These findings on the interactions of two-dimensional nanomaterials and biological systems, along with the enquiry of the mechanisms, would provide essential support for further safety evaluation of the biomedical applications of GO. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000345721400044 |
| 公开日期 | 2015-02-03 |
| 源URL | [http://ir.sinano.ac.cn/handle/332007/1837]  |
| 专题 | 苏州纳米技术与纳米仿生研究所_纳米生物医学与安全研究部_戴建武团队 苏州纳米技术与纳米仿生研究所_纳米生物医学与安全研究部_张智军团队 苏州纳米技术与纳米仿生研究所_纳米生物医学与安全研究部_马宏伟团队 |
| 通讯作者 | Chen, YY (陈艳艳) |
| 推荐引用方式 GB/T 7714 | Ma, HW ,Chen, YY ,Zhang, ZJ . In Vitro Hemocompatibility and Toxic Mechanism of Graphene Oxide on Human Peripheral Blood T Lymphocytes and Serum Albumin[J]. ACS APPLIED MATERIALS & INTERFACES,2014,6(22):19797-19807. |
| APA | Ma, HW ,Chen, YY ,&Zhang, ZJ .(2014).In Vitro Hemocompatibility and Toxic Mechanism of Graphene Oxide on Human Peripheral Blood T Lymphocytes and Serum Albumin.ACS APPLIED MATERIALS & INTERFACES,6(22),19797-19807. |
| MLA | Ma, HW ,et al."In Vitro Hemocompatibility and Toxic Mechanism of Graphene Oxide on Human Peripheral Blood T Lymphocytes and Serum Albumin".ACS APPLIED MATERIALS & INTERFACES 6.22(2014):19797-19807. |
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