A collagen-binding EGFR single-chain Fv antibody fragment for the targeted cancer therapy
文献类型：期刊论文
| 作者 | Liang, H(梁辉); Li, XR(李晓然); Chen, B; Wang, B; Zhao, YN; Zhuang, Y(庄燕); Shen, H(沈贺); Zhang, ZJ(张智军); Dai, JW(戴建武) |
| 刊名 | JOURNAL OF CONTROLLED RELEASE
 |
| 出版日期 | 2015 |
| 卷号 | 209页码:9 |
| 关键词 | Collagen Collagen-binding domain CBD-scFv Controlled release A431 xenograft |
| 通讯作者 | Dai, JW (戴建武) |
| 英文摘要 | Collagen, a primary component of the extracellular matrix (ECM), is highly expressed in a variety of cancers and influences the tumor microenvironment by increasing the recruitment of macrophages and endothelial cells. Therefore, collagen is a highly promising target for cancer therapy. The collagen-binding domain (CBD) can dynamically bind to collagen and achieve the sustained release of CBD-fused protein in the collagen network. Here, we developed a collagen-binding epidermal growth factor receptor (EGFR) antibody fragment for targeting the collagen-rich ECM in tumors. The single chain fragment variable (scFv) of cetuximab was fused to CBD (CBD-scFv) and expressed in Pichia pastoris. CBD-scFv preserved the antigen binding domain and anti-tumor activity of cetuximab in vitro. Moreover, CBD-scFv displayed a collagen binding ability due to the function of CBD. In vivo experiments revealed that CBD-scFv bound to collagen and achieved sustained release in tumors. Furthermore, CBD-scFv significantly suppressed the growth of tumors in A431 xenografts. Therefore, CBD-scFv had a potential therapeutic value for the collagen-rich carcinomas. The specific target and sustained release of CBD-scFv in tumors could be a new approach for targeted drug delivery in cancer therapy. (C) 2015 Elsevier B.V. All rights reserved. |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2016-05-03 |
| 源URL | [http://ir.sinano.ac.cn/handle/332007/3340]  |
| 专题 | 苏州纳米技术与纳米仿生研究所_纳米生物医学与安全研究部_戴建武团队 |
| 推荐引用方式 GB/T 7714 | Liang, H,Li, XR,Chen, B,et al. A collagen-binding EGFR single-chain Fv antibody fragment for the targeted cancer therapy[J]. JOURNAL OF CONTROLLED RELEASE,2015,209:9. |
| APA | Liang, H.,Li, XR.,Chen, B.,Wang, B.,Zhao, YN.,...&Dai, JW.(2015).A collagen-binding EGFR single-chain Fv antibody fragment for the targeted cancer therapy.JOURNAL OF CONTROLLED RELEASE,209,9. |
| MLA | Liang, H,et al."A collagen-binding EGFR single-chain Fv antibody fragment for the targeted cancer therapy".JOURNAL OF CONTROLLED RELEASE 209(2015):9. |
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