中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
A collagen-binding EGFR single-chain Fv antibody fragment for the targeted cancer therapy

文献类型:期刊论文

作者Liang, H(梁辉); Li, XR(李晓然); Chen, B; Wang, B; Zhao, YN; Zhuang, Y(庄燕); Shen, H(沈贺); Zhang, ZJ(张智军); Dai, JW(戴建武)
刊名JOURNAL OF CONTROLLED RELEASE
出版日期2015
卷号209页码:9
关键词Collagen Collagen-binding domain CBD-scFv Controlled release A431 xenograft
通讯作者Dai, JW (戴建武)
英文摘要Collagen, a primary component of the extracellular matrix (ECM), is highly expressed in a variety of cancers and influences the tumor microenvironment by increasing the recruitment of macrophages and endothelial cells. Therefore, collagen is a highly promising target for cancer therapy. The collagen-binding domain (CBD) can dynamically bind to collagen and achieve the sustained release of CBD-fused protein in the collagen network. Here, we developed a collagen-binding epidermal growth factor receptor (EGFR) antibody fragment for targeting the collagen-rich ECM in tumors. The single chain fragment variable (scFv) of cetuximab was fused to CBD (CBD-scFv) and expressed in Pichia pastoris. CBD-scFv preserved the antigen binding domain and anti-tumor activity of cetuximab in vitro. Moreover, CBD-scFv displayed a collagen binding ability due to the function of CBD. In vivo experiments revealed that CBD-scFv bound to collagen and achieved sustained release in tumors. Furthermore, CBD-scFv significantly suppressed the growth of tumors in A431 xenografts. Therefore, CBD-scFv had a potential therapeutic value for the collagen-rich carcinomas. The specific target and sustained release of CBD-scFv in tumors could be a new approach for targeted drug delivery in cancer therapy. (C) 2015 Elsevier B.V. All rights reserved.
收录类别SCI
语种英语
公开日期2016-05-03
源URL[http://ir.sinano.ac.cn/handle/332007/3340]  
专题苏州纳米技术与纳米仿生研究所_纳米生物医学与安全研究部_戴建武团队
推荐引用方式
GB/T 7714
Liang, H,Li, XR,Chen, B,et al. A collagen-binding EGFR single-chain Fv antibody fragment for the targeted cancer therapy[J]. JOURNAL OF CONTROLLED RELEASE,2015,209:9.
APA Liang, H.,Li, XR.,Chen, B.,Wang, B.,Zhao, YN.,...&Dai, JW.(2015).A collagen-binding EGFR single-chain Fv antibody fragment for the targeted cancer therapy.JOURNAL OF CONTROLLED RELEASE,209,9.
MLA Liang, H,et al."A collagen-binding EGFR single-chain Fv antibody fragment for the targeted cancer therapy".JOURNAL OF CONTROLLED RELEASE 209(2015):9.

入库方式: OAI收割

来源:苏州纳米技术与纳米仿生研究所

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