Incorporation of electrospun nanofibrous PVDF membranes into a microfluidic chip assembled by PDMS and scotch tape for immunoassays
文献类型：期刊论文
| 作者 | Liu YY ; Yang DY(仰大勇) ; Yu T ; Jiang XY(蒋兴宇) |
| 刊名 | ELECTROPHORESIS
 |
| 出版日期 | 2009-09 |
| 卷号 | 30期号:18页码:3269-3275 |
| 关键词 | Cross-array Electrospinning Immunoassay Microfluidics PVDF |
| 通讯作者 | Jiang XY(蒋兴宇) |
| 合作状况 | 其它 |
| 英文摘要 | This paper demonstrates a microfluidic chip for multiple immunoassays on electrospun (ES) nanofibrous membranes made of PVDF. This microchip enables the detection of multiple pairs of protein-protein interactions in one experiment by crossing parallel arrays of microchannels. This chip uses ESPVDF membrane with high specific surface area as the substrate to adsorb protein for immobilized immunoassays so that increased levels of protein adsorption may lower the LOD in immunoassays. We employ a simple and effective method for chip assembly by scotch tape to incorporate the ESPVDF membrane into the microchip. We believe that the present work will facilitate the development of methods for the on-site diagnosis of diseases whose detection is based on antigen-antibody recognition, and improve the efficiencies of certain experiments that need to identify tens to hundreds of protein-protein interactions with relatively low cost in the future. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000270446200022 |
| 公开日期 | 2010-01-15 |
| 源URL | [http://58.210.77.100/handle/332007/145]  |
| 专题 | 苏州纳米技术与纳米仿生研究所_纳米生物医学与安全研究部_马宏伟团队 |
| 推荐引用方式 GB/T 7714 | Liu YY,Yang DY,Yu T,et al. Incorporation of electrospun nanofibrous PVDF membranes into a microfluidic chip assembled by PDMS and scotch tape for immunoassays[J]. ELECTROPHORESIS,2009,30(18):3269-3275. |
| APA | Liu YY,Yang DY,Yu T,&Jiang XY.(2009).Incorporation of electrospun nanofibrous PVDF membranes into a microfluidic chip assembled by PDMS and scotch tape for immunoassays.ELECTROPHORESIS,30(18),3269-3275. |
| MLA | Liu YY,et al."Incorporation of electrospun nanofibrous PVDF membranes into a microfluidic chip assembled by PDMS and scotch tape for immunoassays".ELECTROPHORESIS 30.18(2009):3269-3275. |
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