Label-Free Real-Time Detection of DNA Methylation Based on Quartz Crystal Microbalance Measurement
文献类型：期刊论文
| 作者 | Ma, HW(马宏伟)
|
| 刊名 | ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2013-02-19 |
| 卷号 | 85期号:4页码:2096-2101 |
| 通讯作者 | Ma, HW(马宏伟) |
| 英文摘要 | DNA methylation plays an important role in the regulation of gene transcription, chromatin compaction, genome imprinting, and X-chromosome inactivation. DNA methyltransferase is considered a potential target for anticancer drug design. It is important to locate aberrantly methylated sequences on the human genome that are linked to specific diseases and to discover new low-toxic methylation inhibitors for medical treatments. We developed a DNA methylation detection method using a quartz crystal microbalance (QCM). We applied this method to assay genes p16 and GALR2 in two cell lines. Methylation of p16 was detected in both HT29 and HepG2 cell lines, whereas methylation of GALR2 was detected only in the HT29 cell line. We also used this method to evaluate the effect of 5-aza-2'-deoxycytidine (decitabine), a methyltransferase inhibitor used in clinical treatment. We found methylation of genes p16 and GALR2 to be strongly inhibited. The results show that this method is sensitive to DNA methylation and is fit for evaluation of methyltransferase inhibitors. |
| 收录类别 | SCI ; EI |
| 语种 | 英语 |
| WOS记录号 | WOS:000315326900022 |
| 公开日期 | 2014-01-14 |
| 源URL | [http://ir.sinano.ac.cn/handle/332007/1347]  |
| 专题 | 苏州纳米技术与纳米仿生研究所_纳米生物医学与安全研究部_马宏伟团队 |
| 通讯作者 | Ma, HW(马宏伟) |
| 推荐引用方式 GB/T 7714 | Ma, HW. Label-Free Real-Time Detection of DNA Methylation Based on Quartz Crystal Microbalance Measurement[J]. ANALYTICAL CHEMISTRY,2013,85(4):2096-2101. |
| APA | Ma, HW.(2013).Label-Free Real-Time Detection of DNA Methylation Based on Quartz Crystal Microbalance Measurement.ANALYTICAL CHEMISTRY,85(4),2096-2101. |
| MLA | Ma, HW."Label-Free Real-Time Detection of DNA Methylation Based on Quartz Crystal Microbalance Measurement".ANALYTICAL CHEMISTRY 85.4(2013):2096-2101. |
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