Simultaneous and rapid detection of multiple pesticide and veterinary drug residues by suspension array technology
文献类型：期刊论文
| 作者 | Ma, HW(马宏伟)
|
| 刊名 | BIOSENSORS & BIOELECTRONICS
 |
| 出版日期 | 2013-03-15 |
| 卷号 | 41期号:0页码:710-716 |
| 关键词 | Suspension array Microsphere/bead Residue Median fluorescent intensity (MFI) |
| 通讯作者 | Gao, ZX |
| 英文摘要 | Suspension array technology is proposed for the simultaneous quantitative determination of seven kinds of pesticide and veterinary drug residues, namely, atrazine, chloramphenicol, carbaryl, clenbuterol, 17-beta-estradiol, imidacloprid, and tylosin. The assay is simple and can be accomplished within 2 h without repeated pumping and washing steps unlike conventional suspension arrays. The hapten-protein conjugate-coated beads bind to their complementary biotinylated antibodies using a competitive immunoassay format. The coefficients of determination R-2 for six targets were greater than 0.992, whereas that for atrazine was 0.961, which indicate good logistic correlations. The dynamic ranges for the seven targets in the 7-plex assay ranged from 2 log units to 4 log units(1.60 x 10(0)-1.64 x 10(3), 5.12 x 10(-2)-1.60 x 10(2), 1.00 x 10(0)-3.13 x 10(3), 4.00 x 10(-1)-4.10 x 10(2), 4.00 x 10(-1)-4.10 x 10(2), 5.12 x 10(-2)-1.60 x 10(2), and 2.00 x 10(0)-4.00 x 10(2) ng mL(-1)). The minimum detection concentrations of chloramphenicol, carbaryl, clenbuterol and 17-beta-estradiol in the suspension array (0.05, 1.00, 0.40 and 0.40 ng mL(-1)) were lower than the corresponding limits of detection (0.25, 6.60, 24.23 and 13.96 ng mL(-1)) of using an indirect competitive enzyme-linked immunosorbent assay. Environmental scanning electron microscope was employed to characterize the bead surface, which directly confirmed the reactions on the beads. The suspension array is more flexible and feasible than ELISA for the fast quantitative analysis of pesticide and veterinary drug residues. |
| 收录类别 | SCI ; EI |
| 语种 | 英语 |
| WOS记录号 | WOS:000314191300106 |
| 公开日期 | 2014-01-15 |
| 源URL | [http://ir.sinano.ac.cn/handle/332007/1411]  |
| 专题 | 苏州纳米技术与纳米仿生研究所_纳米生物医学与安全研究部_马宏伟团队 |
| 推荐引用方式 GB/T 7714 | Ma, HW. Simultaneous and rapid detection of multiple pesticide and veterinary drug residues by suspension array technology[J]. BIOSENSORS & BIOELECTRONICS,2013,41(0):710-716. |
| APA | Ma, HW.(2013).Simultaneous and rapid detection of multiple pesticide and veterinary drug residues by suspension array technology.BIOSENSORS & BIOELECTRONICS,41(0),710-716. |
| MLA | Ma, HW."Simultaneous and rapid detection of multiple pesticide and veterinary drug residues by suspension array technology".BIOSENSORS & BIOELECTRONICS 41.0(2013):710-716. |
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