Label-free detection of T4 DNA ligase and polynucleotide kinase activity based on toehold-mediated strand displacement and split G-quadruplex probes
文献类型：期刊论文
| 作者 | Guo, YH(郭亚辉); Wang, QL(王庆琳); Wang, ZL(王志利); Chen, X(陈星); Xu, LJ(许丽君); Hu, JM; Pei, RJ(裴仁军) |
| 刊名 | SENSORS AND ACTUATORS B-CHEMICAL
 |
| 出版日期 | 2015 |
| 卷号 | 214页码:6 |
| 关键词 | DNA ligase Polynucleotide kinase Label free Toehold-mediated strand displacement Split G-quadruplex |
| 通讯作者 | Pei, RJ (裴仁军) |
| 英文摘要 | Herein, an efficient pair of split G-quadruplex probes was selected (S/N= 21) to construct a simple, rapid and label-free technique for the detections of T4 DNA ligase and PNK activity. DNA ligase and PNK are engaged in DNA replication and repair, ensuring the genetic stability. The detections of T4 ligase and PNK activity are of great significance in biology and biomedicine. Split G-quadruplex probes are robust label-free biosensing tools with turn-on signal readout. The selected pair of split G-quadruplex probes combined with toehold-mediated strand displacement in this work demonstrated an efficient strategy for monitoring the activities of T4 DNA ligase and PNK with good sensitivity. (C) 2015 Elsevier B.V. All rights reserved. |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2016-05-03 |
| 源URL | [http://ir.sinano.ac.cn/handle/332007/3335]  |
| 专题 | 苏州纳米技术与纳米仿生研究所_纳米生物医学与安全研究部_裴仁军团队 |
| 推荐引用方式 GB/T 7714 | Guo, YH,Wang, QL,Wang, ZL,et al. Label-free detection of T4 DNA ligase and polynucleotide kinase activity based on toehold-mediated strand displacement and split G-quadruplex probes[J]. SENSORS AND ACTUATORS B-CHEMICAL,2015,214:6. |
| APA | Guo, YH.,Wang, QL.,Wang, ZL.,Chen, X.,Xu, LJ.,...&Pei, RJ.(2015).Label-free detection of T4 DNA ligase and polynucleotide kinase activity based on toehold-mediated strand displacement and split G-quadruplex probes.SENSORS AND ACTUATORS B-CHEMICAL,214,6. |
| MLA | Guo, YH,et al."Label-free detection of T4 DNA ligase and polynucleotide kinase activity based on toehold-mediated strand displacement and split G-quadruplex probes".SENSORS AND ACTUATORS B-CHEMICAL 214(2015):6. |
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