One-Pot Polymerase Chain Reaction with Gold Nanoparticles for Rapid and Ultrasensitive DNA Detection
文献类型：期刊论文
| 作者 | Cai MA (蔡苗); Li F (李峰); Zhang Y (张岩); Wang QB (王强斌)
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| 刊名 | Nano Research
 |
| 出版日期 | 2010-08 |
| 卷号 | 3期号:8页码:557-563 |
| 关键词 | deoxyribonucleic acid ( DNA) detection polymerase chain reaction (PCR) Au nanoparticle colorimetric |
| 通讯作者 | Wang QB (王强斌) |
| 合作状况 | 其它 |
| 英文摘要 | We report a novel method for rapid, colorimetric detection of a specific deoxyribonucleic acid ( DNA) sequence by carrying out a polymerase chain reaction in the presence of gold nanoparticles functionalized with two primers. Extension of the primers when the target DNA is present as a template during the polymerase chain reaction process affords the complementary sequences on the gold nanoparticle surfaces and results in the formation of gold nanoparticle aggregates with a concomitant color change from red to pinkish/purple. This method provides a convenient and straightforward solution for ultrasensitive DNA detection without any further post-treatment of the polymerase chain reaction products being necessary, and is a promising tool for rapid disease diagnostics and gene sequencing. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000280825000003 |
| 公开日期 | 2011-03-14 |
| 源URL | [http://58.210.77.100/handle/332007/368]  |
| 专题 | 苏州纳米技术与纳米仿生研究所_纳米生物医学与安全研究部_王强斌团队 |
| 推荐引用方式 GB/T 7714 | Cai MA ,Li F ,Zhang Y ,et al. One-Pot Polymerase Chain Reaction with Gold Nanoparticles for Rapid and Ultrasensitive DNA Detection[J]. Nano Research,2010,3(8):557-563. |
| APA | Cai MA ,Li F ,Zhang Y ,&Wang QB .(2010).One-Pot Polymerase Chain Reaction with Gold Nanoparticles for Rapid and Ultrasensitive DNA Detection.Nano Research,3(8),557-563. |
| MLA | Cai MA ,et al."One-Pot Polymerase Chain Reaction with Gold Nanoparticles for Rapid and Ultrasensitive DNA Detection".Nano Research 3.8(2010):557-563. |
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