Quantum dot-induced viral capsid assembling in dissociation buffer
文献类型：期刊论文
| 作者 | Gao, D; Zhang, ZP ; Li, F(李峰); Men, D; Deng, JY; Wei, HP; Zhang, XE; Cui, ZQ
|
| 刊名 | INTERNATIONAL JOURNAL OF NANOMEDICINE
 |
| 出版日期 | 2013 |
| 卷号 | 8期号:0页码:2119-2128 |
| 关键词 | quantum dots simian virus 40 self-assembly encapsulation virus-based nanoparticles |
| 通讯作者 | Zhang, XE |
| 英文摘要 | Viruses encapsulating inorganic nanoparticles are a novel type of nanostructure with applications in biomedicine and biosensors. However, the encapsulation and assembly mechanisms of these hybridized virus-based nanoparticles (VNPs) are still unknown. In this article, it was found that quantum dots (QDs) can induce simian virus 40 (SV40) capsid assembly in dissociation buffer, where viral capsids should be disassembled. The analysis of the transmission electron microscope, dynamic light scattering, sucrose density gradient centrifugation, and cryo-electron microscopy single particle reconstruction experimental results showed that the SV40 major capsid protein 1 (VP1) can be assembled into approximate to 25 nm capsids in the dissociation buffer when QDs are present and that the QDs are encapsulated in the SV40 capsids. Moreover, it was determined that there is a strong affinity between QDs and the SV40 VP1 proteins (K-D = 2.19E-10 M), which should play an important role in QD encapsulation in the SV40 viral capsids. This study provides a new understanding of the assembly mechanism of SV40 virus-based nanoparticles with QDs, which may help in the design and construction of other similar virus-based nanoparticles. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000319901300001 |
| 公开日期 | 2014-01-20 |
| 源URL | [http://ir.sinano.ac.cn/handle/332007/1532]  |
| 专题 | 苏州纳米技术与纳米仿生研究所_纳米生物医学与安全研究部_王强斌团队 |
| 推荐引用方式 GB/T 7714 | Gao, D,Zhang, ZP,Li, F,et al. Quantum dot-induced viral capsid assembling in dissociation buffer[J]. INTERNATIONAL JOURNAL OF NANOMEDICINE,2013,8(0):2119-2128. |
| APA | Gao, D.,Zhang, ZP.,Li, F.,Men, D.,Deng, JY.,...&Cui, ZQ.(2013).Quantum dot-induced viral capsid assembling in dissociation buffer.INTERNATIONAL JOURNAL OF NANOMEDICINE,8(0),2119-2128. |
| MLA | Gao, D,et al."Quantum dot-induced viral capsid assembling in dissociation buffer".INTERNATIONAL JOURNAL OF NANOMEDICINE 8.0(2013):2119-2128. |
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