Combination of TNF-alpha and graphene oxide-loaded BEZ235 to enhance apoptosis of PIK3CA mutant colorectal cancer cells
文献类型：期刊论文
| 作者 | Huang, J(黄洁) ; Zhang, ZJ(张智军); Zhu, YM(朱毅敏)
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| 刊名 | JOURNAL OF MATERIALS CHEMISTRY B
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| 出版日期 | 2013 |
| 卷号 | 1期号:41页码:5602-5610 |
| 通讯作者 | Zhang, ZJ(张智军) |
| 英文摘要 | The PI3K–AKT–mTOR pathway plays an important role in tumor cell growth, invasion, migration and apoptosis. A blockade of this signaling pathway has arisen as a compelling target for the tumor therapy. However, there is cross-talking between different signal pathways. Combined treatment of tumors with different signal pathway inhibitors is considered as an efficient strategy for cancer therapy. NVP-BEZ235 is a dual pan-class I PI3K and mTOR kinase inhibitor currently in clinical trial. TNF-α is involved in the regulation of cell apoptosis. In the current work, we explored the combined use of BEZ235 and TNF-α on the PIK3CA mutant colorectal cancer (CRC) cell proliferation inhibition. In our strategy, the BEZ235 is loaded on PEGylated graphene oxide (GO-PEG) by physisorption via π–π stacking to enhance its aqueous solubility. The resulting GO-BEZ235 complex exhibited excellent aqueous solubility while retaining a high cancer cell killing potency. The combination of BEZ235 and TNF-α shows an enhanced cellular proliferation inhibition for HCT 116 through enhancing the G1 phase arrest and cell apoptosis compared to either drug alone. Moreover, our experiments reveal that the enhanced tumor cell apoptosis depends on the activation of caspase-9, caspase-8 and caspase-3 mediated by the increased phosphorylation level of JNK. Taken together, our findings demonstrate for the first time the feasibility of BEZ235 delivered by GO-PEG and of the combined use of BEZ235 and TNF-α for PIK3CA mutant CRC therapy. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000325762800009 |
| 公开日期 | 2014-01-09 |
| 源URL | [http://ir.sinano.ac.cn/handle/332007/1270]  |
| 专题 | 苏州纳米技术与纳米仿生研究所_纳米生物医学与安全研究部_张智军团队 |
| 通讯作者 | Zhang, ZJ(张智军) |
| 推荐引用方式 GB/T 7714 | Huang, J,Zhang, ZJ,Zhu, YM. Combination of TNF-alpha and graphene oxide-loaded BEZ235 to enhance apoptosis of PIK3CA mutant colorectal cancer cells[J]. JOURNAL OF MATERIALS CHEMISTRY B,2013,1(41):5602-5610. |
| APA | Huang, J,Zhang, ZJ,&Zhu, YM.(2013).Combination of TNF-alpha and graphene oxide-loaded BEZ235 to enhance apoptosis of PIK3CA mutant colorectal cancer cells.JOURNAL OF MATERIALS CHEMISTRY B,1(41),5602-5610. |
| MLA | Huang, J,et al."Combination of TNF-alpha and graphene oxide-loaded BEZ235 to enhance apoptosis of PIK3CA mutant colorectal cancer cells".JOURNAL OF MATERIALS CHEMISTRY B 1.41(2013):5602-5610. |
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