The HGF inhibitory peptide HGP-1 displays promising in vitro and in vivo efficacy for targeted cancer therapy
文献类型：期刊论文
| 作者 | Chen, LS(陈丽莎); Li, CL(李春林); Zhu, YM(朱毅敏)
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| 刊名 | ONCOTARGET
 |
| 出版日期 | 2015 |
| 卷号 | 6期号:30页码:14 |
| 关键词 | HGF targeting peptide HGF/MET signaling pathway cancer targeted therapy |
| 通讯作者 | Zhu, YM (朱毅敏) |
| 英文摘要 | HGF/MET pathway mediates cancer initiation and development. Thus, inhibition on HGF-initiated MET signaling pathway would provide a new approach to cancer targeted therapeutics. In our study, we identified a targeting peptide candidate binding to HGF which was named HGF binding peptide-1 (HGP-1) via bacterial surface display methods coupled with fluorescence-activated cell sorting (FACS). HGP-1 showed the moderate affinity when determined with surface plasmon resonance (SPR) technique and high specificity in binding to HGF while assessed by fluorescence-based ELISA assay. The results from MTT and in vitro migration assay indicated that HGF-dependent cell proliferation and migration could be inhibited by HGP-1. In vivo administration of HGP-1 led to an effective inhibitory effect on tumor growth in A549 tumor xenograft models. Moreover, findings from Western Blots revealed that HGP-1 could down-regulated the phosphorylation levels of MET and ERK1/2 initiated by HGF, which suggested that HGP-1 could disrupt the activation of HGF/MET signaling to influence the cell activity. All the data highlighted the potential of HGP-1 to be a potent inhibitor for HGF/MET signaling. |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2016-05-03 |
| 源URL | [http://ir.sinano.ac.cn/handle/332007/3294]  |
| 专题 | 苏州纳米技术与纳米仿生研究所_纳米生物医学与安全研究部_朱毅敏团队 |
| 推荐引用方式 GB/T 7714 | Chen, LS,Li, CL,Zhu, YM. The HGF inhibitory peptide HGP-1 displays promising in vitro and in vivo efficacy for targeted cancer therapy[J]. ONCOTARGET,2015,6(30):14. |
| APA | Chen, LS,Li, CL,&Zhu, YM.(2015).The HGF inhibitory peptide HGP-1 displays promising in vitro and in vivo efficacy for targeted cancer therapy.ONCOTARGET,6(30),14. |
| MLA | Chen, LS,et al."The HGF inhibitory peptide HGP-1 displays promising in vitro and in vivo efficacy for targeted cancer therapy".ONCOTARGET 6.30(2015):14. |
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