Tri-component nanostructures of C₆₀/m-xylene/thiophene were synthesized via a solvent-induced self-assembly method and characterized by SEM, TEM, XRD, FT-IR, and Raman. The shapes of nanostructures were dependent on the concentration of C₆₀, the volume ratio of thiophene to m-xylene, and the volume ratio of mixture solvent to isopropanol. High-resolution TEM image and SAED pattern revealed that twisted nanorods of C₆₀ were single crystals and had an hcp structure. The EDX and FT-IR analysis confirmed the existence of thiophene. The results indicated that the twisted nanorods consisted of pristine C₆₀, m-xylene, and thiophene molecules. In addition, thiophene molecules were assumed to partially replace m-xylene molecule in the C₆₀/m-xylene lattice. Such tri-component nanorods of C₆₀ are ideal building blocks for fabricating novel nanodevices.