Demethylation of c-MYB binding site mediates upregulation of Bdnf IV in cocaine-conditioned place preference
文献类型：期刊论文
| 作者 | Tian, Weiping1,2; Wang JS(王杰思)1,3 ; Zhang, Ke1; Teng, Huajing3; Li, Chong1; Szyf, Moshe5; Sun, Zhong Sheng3,4; Zhao, Mei1
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| 刊名 | SCIENTIFIC REPORTS
 |
| 出版日期 | 2016-02-25 |
| 卷号 | 6期号:22页码:087 |
| ISSN号 | 2045-2322 |
| 英文摘要 | Abnormal BDNF signaling contributes to the structural and behavioral plasticity induced by drugs of abuse. However, the mechanisms regulating expression of Bdnf in drug addiction remain elusive. In the present study, using the conditioned place preference (CPP) model, we showed that expression of Bdnf IV is upregulated in the nucleus accumbens (NAc) of conditioned animals while Bdnf I is upregulated in cocaine-treated mice irrespective of conditioning. The methylation level of a putative c-MYB binding site in the promoter region of Bdnf IV was significantly decreased in the NAc under cocaine CPP conditioning but remained unchanged without conditioning, concurrently with increased binding of c-MYB to this site. Exon IV promoter/luciferase reporter assays revealed that transactivation of Bdnf by c-MYB was blocked by methylation of this c-MYB binding site. Administration of methionine, a precursor of SAM, inhibited cocaine CPP, reversed demethylation of c-MYB binding site and induction of Bdnf IV expression by cocaine CPP. Our results imply that Bdnf IV demethylation at c-MYB binding site is involved in cocaine-triggered seeking behavior, whereas Bdnf I responds to the immediate pharmacological effects of cocaine. |
| WOS标题词 | Science & Technology |
| 类目[WOS] | Multidisciplinary Sciences |
| 研究领域[WOS] | Science & Technology - Other Topics |
| 关键词[WOS] | MESOLIMBIC DOPAMINE SYSTEM ; NEUROTROPHIC FACTOR ; DNA METHYLATION ; TRANSCRIPTION FACTOR ; DRUG-ADDICTION ; EPIGENETIC REGULATION ; NUCLEUS-ACCUMBENS ; SPLICE VARIANTS ; BRAIN ; EXPRESSION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000370800800002 |
| 源URL | [http://ir.psych.ac.cn/handle/311026/19586]  |
| 专题 | 心理研究所_中国科学院心理健康重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Psychol, Key Lab Mental Hlth, 16 Lincui Rd, Beijing 100101, Peoples R China 2.Tianjin Med Univ, Tianjin Res Ctr Basic Med Sci, Tianjin 300070, Peoples R China 3.Chinese Acad Sci, Beijing Inst Life Sci, Beijing 100101, Peoples R China 4.Univ Town, Wenzhou Med Coll, Inst Genom Med, Wenzhou 325000, Zhejiang, Peoples R China 5.McGill Univ, Dept Pharmacol & Therapeut, Montreal, PQ H3G 1Y6, Canada |
| 推荐引用方式 GB/T 7714 | Tian, Weiping,Wang JS,Zhang, Ke,et al. Demethylation of c-MYB binding site mediates upregulation of Bdnf IV in cocaine-conditioned place preference[J]. SCIENTIFIC REPORTS,2016,6(22):087. |
| APA | Tian, Weiping.,Wang JS.,Zhang, Ke.,Teng, Huajing.,Li, Chong.,...&Zhao, Mei.(2016).Demethylation of c-MYB binding site mediates upregulation of Bdnf IV in cocaine-conditioned place preference.SCIENTIFIC REPORTS,6(22),087. |
| MLA | Tian, Weiping,et al."Demethylation of c-MYB binding site mediates upregulation of Bdnf IV in cocaine-conditioned place preference".SCIENTIFIC REPORTS 6.22(2016):087. |
入库方式： OAI收割
来源：心理研究所
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