A Label-Free Electrochemical Impedance Cytosensor Based on Specific Peptide-Fused Phage Selected from Landscape Phage Library
文献类型：期刊论文
| 作者 | Han, Lei1,2,3 ; Liu, Pei3; Petrenko, Valery A.4; Liu, Aihua1,2,3
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| 刊名 | SCIENTIFIC REPORTS
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| 出版日期 | 2016-02-24 |
| 卷号 | 6 |
| 英文摘要 | One of the major challenges in the design of biosensors for cancer diagnosis is to introduce a low-cost and selective probe that can recognize cancer cells. In this paper, we combined the phage display technology and electrochemical impedance spectroscopy (EIS) to develop a label-free cytosensor for the detection of cancer cells, without complicated purification of recognition elements. Fabrication steps of the cytosensing interface were monitored by EIS. Due to the high specificity of the displayed octapeptides and avidity effect of their multicopy display on the phage scaffold, good biocompatibility of recombinant phage, the fibrous nanostructure of phage, and the inherent merits of EIS technology, the proposed cytosensor demonstrated a wide linear range (2.0 x 10(2) - 2.0 x 10(8) cells mL(-1)), a low limit of detection (79 cells mL(-1), S/N = 3), high specificity, good inter-and intra-assay reproducibility and satisfactory storage stability. This novel cytosensor designing strategy will open a new prospect for rapid and label-free electrochemical platform for tumor diagnosis. |
| WOS标题词 | Science & Technology |
| 类目[WOS] | Multidisciplinary Sciences |
| 研究领域[WOS] | Science & Technology - Other Topics |
| 关键词[WOS] | SURFACE-PLASMON RESONANCE ; COVALENT VIRUS LAYER ; IMPEDIMETRIC SENSOR ; CANCER-CELLS ; LUNG-CANCER ; SPECTROSCOPY ; DISPLAY ; BACTERIA ; APTAMER ; CARBOHYDRATE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000370773500001 |
| 公开日期 | 2016-05-03 |
| 源URL | [http://ir.qibebt.ac.cn/handle/337004/8018]  |
| 专题 | 青岛生物能源与过程研究所_生物传感技术团队 |
| 作者单位 | 1.Qingdao Univ, Inst Biosensing & Invitro Diagnost, 38 Dengzhou Rd, Qingdao 266021, Peoples R China 2.Qingdao Univ, Coll Med, 38 Dengzhou Rd, Qingdao 266021, Peoples R China 3.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Lab Biosensing, 189 Songling Rd, Qingdao 266101, Peoples R China 4.Auburn Univ, Coll Vet Med, Dept Pathobiol, 269 Greene Hall, Auburn, AL 36849 USA |
| 推荐引用方式 GB/T 7714 | Han, Lei,Liu, Pei,Petrenko, Valery A.,et al. A Label-Free Electrochemical Impedance Cytosensor Based on Specific Peptide-Fused Phage Selected from Landscape Phage Library[J]. SCIENTIFIC REPORTS,2016,6. |
| APA | Han, Lei,Liu, Pei,Petrenko, Valery A.,&Liu, Aihua.(2016).A Label-Free Electrochemical Impedance Cytosensor Based on Specific Peptide-Fused Phage Selected from Landscape Phage Library.SCIENTIFIC REPORTS,6. |
| MLA | Han, Lei,et al."A Label-Free Electrochemical Impedance Cytosensor Based on Specific Peptide-Fused Phage Selected from Landscape Phage Library".SCIENTIFIC REPORTS 6(2016). |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
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