Molecularly imprinted polymer for the specific solid-phase extraction of kirenol from Siegesbeckia pubescens herbal extract
文献类型：期刊论文
| 作者 | Chen FF(陈方方)1,2,3; Wang R(王瑞)1,2 ; Shi YP(师彦平)1,2; Shi YP(师彦平)
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| 刊名 | Talanta
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| 出版日期 | 2012 |
| 卷号 | 89页码:505-512 |
| ISSN号 | 0039-9140 |
| 关键词 | Molecularly imprinted polymers Solid phase extraction Kirenol Diterpenoids Traditional chinese medicines |
| 通讯作者 | 师彦平 |
| 英文摘要 | Molecular imprinted polymers (MIPs) were prepared by thermal polymerization using a non-covalent molecularly imprinting strategy with kirenol as the template, acrylamide (AM) as the functional monomer and ethylene glycol dimethacrylamide (EGDMA) as the cross-linker in the porogen of tetrahydrofuran (THF). The synthesized MIPs were characterized by scanning electron microscopy (SEM) and Fourier transform infrared (FT-IR). Its molecular recognition property was investigated by UV spectrogram. High-pressure liquid chromatography (HPLC) was used for analysis of target analytes. The polymers were evaluated further by batch rebinding experiments, and from the derived isotherms their binding capacity and binding strength were determined. Then the selectivity of the MIPs was checked toward the selected structurally related compounds and the recognition coefficients for kirenol, darutigenol, and ent-2-oxo-15, 16, 19-trihydroxypimar-8(14)-ene (TD) were 2.47, 3.43 and 3.40, respectively. The properties of MIPs for SPE were also evaluated. The results obtained demonstrate that the good imprinting effect and the excellent selectivity of MIPs were obtained. The optimized molecular imprinted SPE procedure was applied to extract kirenol directly from the extracts of the aerial part of Siegesbeckia pubescens herb. A selective extraction of kirenol from traditional Chinese medicine (TCM) was achieved with extraction yield of 80.9%. |
| 学科主题 | 分析化学与药物化学 |
| 资助信息 | National Natural Science Foundation of China (Nos. 21075127;20875095) |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000301278000073 |
| 公开日期 | 2016-05-03 |
| 源URL | [http://210.77.64.217/handle/362003/19503]  |
| 专题 | 兰州化学物理研究所_中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
| 通讯作者 | Shi YP(师彦平) |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Chem NW Plant Resources, Lanzhou Inst Chem Phys, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Key Lab Nat Med Gansu Prov, Lanzhou Inst Chem Phys, Lanzhou 730000, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen FF,Wang R,Shi YP,et al. Molecularly imprinted polymer for the specific solid-phase extraction of kirenol from Siegesbeckia pubescens herbal extract[J]. Talanta,2012,89:505-512. |
| APA | Chen FF,Wang R,Shi YP,&师彦平.(2012).Molecularly imprinted polymer for the specific solid-phase extraction of kirenol from Siegesbeckia pubescens herbal extract.Talanta,89,505-512. |
| MLA | Chen FF,et al."Molecularly imprinted polymer for the specific solid-phase extraction of kirenol from Siegesbeckia pubescens herbal extract".Talanta 89(2012):505-512. |
入库方式： OAI收割
来源：兰州化学物理研究所
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